Words similar to nacl
Example sentences for: nacl
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The inhibitory activities of fibrosarcoma PAI-1, wtPAI-1, and its mutants were measured by a direct chromogenic assay using uPA (100 U/ug) and its substrate S-2444 in a microtiter plate as described earlier [ 20 ] . Briefly, fibrosarcoma PAI-1, wtPAI-1, or its mutants were diluted 1:10 in the activity measuring assay buffer (50 uM Tris-HCl, pH 7.5, containing 0.15 M NaCl and 100 ug/ml BSA).
Sections were treated with RNase A (10 μg/ml) at 37°C for 30 minutes, followed by washes in 10 mM Tris, 5 mM EDTA, 500 mM NaCl at 37°C; 2X SSC at 50°C; and 0.1X SSC at 50°C.
The Kreb's solution consisted of (mM): NaCl 112, KCl 4.7, MgSO 4 1.2, Na-EDTA 0.5, NaHCO 3 28.
To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.
7, 1 M NaCl, 0.01% Triton, 0.5 mg/ml BSA, 0.1 mg/ml H. Sperm DNA, 50 pM Control Oligo B2, and 1X Eukaryotic hybridization Control) was added to each sample.
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