Words similar to nacl

Example sentences for: nacl

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After washing twice with 3 ml of plain medium, the cells were lysed with 0.2 ml of lysis buffer containing 0.1 M NaCl, 0.01 M EDTA, 0.3 M Tris-HCl (pH 8.0), 0.2 M sucrose and 0.5% SDS.
The membranes were blocked for 1 h at room temperature with 5% nonfat milk in Tris-buffered saline (TBS: 100 mM Tris, 0.9% NaCl, pH 7.5) containing 0.1% Tween 20, then incubated overnight at 4°C with primary antibody diluted in the blocking solution.
Third instar wandering larvae were dissected and washed several times in Ringer solution (130 mM NaCl, 5 mM KCl, 2 mM Na 2 HPO 4 , 1.5 mM CaCl 2 , 0.37 mM KH 2 PO 4 ). Fat-body fragments were mainly anterior plates.
0.25 mM EDTA) for 10 min at 37°C, and then with 400 μl collagenase D (5 mg/ml; Boehringer) in collagenase buffer (20 mM Hepes pH 7, 0.13 mM NaCl, 10 mM Ca Acetate) for 30 min at 37°C.
Four types of media containing 2% granulated agar (Difco, Detroit, MI) were used to grow fungi: (1) potato dextrose agar [ 31 ] , (2) potato sucrose agar (similar to potato dextrose agar except sucrose is used instead of dextrose; [ 31 ] , (3) casein agar (for 1 L, 5 g casein, 0.5 g NaCl, 20 g sucrose, and 20 g agar), and (4) sagebrush-leaf agar.