Example sentences for: nacl

How can you use “nacl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 9, 300 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT, and 1 mM PMSF).

  • The cells were preincubated for 20 minutes in a choline-free physiological salt solution (pH 7.4) consisting of 135 mM NaCl, 5 mM KCl, 1 mM CaCl 2 , 0.75 mM MgCl 2 , 10 mM glucose and 10 mM HEPES.

  • 5 mM MgCl 2 and stored in viral preservation medium (25% glycerol/5 mM Tris pH8/50 mM NaCl/0.

  • Precipiates were washed four times in 20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMS, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.

  • As a result, Top67 could position itself in the absence of ZD domain at the junction of double- and single-stranded DNA for subsequent DNA cleavage, as previously observed for intact topoisomerase I [ 8 ] . Reversal of DNA cleavage also took place readily with Top67 upon addition of 1 M NaCl and 4 mM MgCl 2 . The ZD domain also was not required for selectivity of a cytosine in the -4 position relative to the cleavage sites.


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