Words similar to nacl

Example sentences for: nacl

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Adhesion assays were performed in modified Tyrodes buffer [ 30 ] composed of 12 mM NaHCO 3 (pH 7.4), 150 mM NaCl, 2.5 mM KCl, 2 mM MgCl 2 , 1 mg/ml BSA, and 1 mg/ml glucose.
Binding of β-glucuronidase was assayed as described previously [ 31 32 ] . Briefly, cells were permeabilized with 0.25% saponin in 50 mM Hepes (pH 7.0), 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, and 10 mM mannose-6-phosphate (M6P) for 30 minutes on ice.
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
We observed that more complete and consistent reversal of cleavage was obtained with both topoisomerase I and Top67 if a low concentration of Mg 2+(4 mM) was also added with the NaCl.
4Dand 4E), although cytoplasmic staining was also observed, particularly in cells of S rats on the 8.0% NaCl diet.