Words similar to nacl

Example sentences for: nacl

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Transfected cells were transferred to a recording chamber containing extracellular solution (140 mM NaCl, 1.7 mM MgCl 2 , 5 mM KCl, 1.8 mM CaCl 2 , 25 mM HEPES pH 7.4).
The supernatant was assayed for caspase activity in 20 mM Piperazine-N,N'-bis(2-ethanesulfonic acid) (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1% CHAPS, and 10% sucrose with Z-IETD-AFC for caspase 8 [ 42 43 ] . The release of the fluorogenic-leaving group was detected by excitation at 400 nm and emission at 505 nm.
The cells were preincubated for 20 minutes in a choline-free physiological salt solution (pH 7.4) consisting of 135 mM NaCl, 5 mM KCl, 1 mM CaCl 2 , 0.75 mM MgCl 2 , 10 mM glucose and 10 mM HEPES.
The Triton-insoluble fractions were then scraped into cold Triton X-100 lysis buffer [50 mM Tris/HCl (pH 7.4); 100 mM NaCl; 50 mM NaF; 5 mM EDTA; 40 mM glycerophosphate; 1 mM sodium orthovanadate; 100 μM PMSF; 1 μM leupeptin; 1 μM pepstatin A; 1% (v/v) Triton X-100] and incubated for 20 min on ice, and clarified by centrifugation at 13000 g for 5 min at 4°C.
After the final incubation step of the protein complete assembly process, beads were extensively washed, followed by a final wash with Kinase Buffer [5 mM Tris·HCl (pH 8.0), 25 mM NaCl, 10 mM MgCl 2 , 1 mM DTT, 0.1 mg/ml BSA, 1 mM ATP, 10 μg/ml pepstatin + chymostatin + leupeptin (PCL)].