Words similar to nacl
Example sentences for: nacl
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The cells were incubated in 0.1 M borate buffer (pH 8.5, 0.1 M boric acid, 25 mM Na 2 B 4 O 7 , and 75 mM NaCl) twice for 5 minutes each (10 minutes total), followed by three 10 minute washes in PBS.
At the time of experimental treatment, the culture media was replaced with Locke's buffer containing (in mM) 154 NaCl, 5.6 KCl, 2.3 CaCl 2 , 1 MgCl 2 , 3.6 NaHCO 3 , 5 glucose, 5 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (pH 7.2) and neurons were incubated with culture supernatants from HIV infected monocytic cells, subtoxic concentrations of Tat (40 nM) + gp120 (30 pM), various concentrations of methamphetamine or cocaine, or viral proteins + methamphetamine and cocaine.
CAP, cellulose acetate phthalate; STD, sexually transmitted disease; ELISA, enzyme-linked immunosorbent assay; mAbs, monoclonal antibodies; FBS, fetal bovine serum; PEG 6000, polyethylene glycol 6000; HIV-1, human immunodeficiency virus type 1; SIV, simian immunodeficiency virus; HSV-1, herpesvirus type 1; HSV-2, herpesvirus type 2; BSA, bovine serum albumin; PBS, phosphate buffered saline; HRP, horse radish peroxidase; sCD4, soluble CD4; TS, 0.14 M NaCl, 0.01 M Tris, pH 7.0; PBL, peripheral blood lymphocytes; CTAP, cellotetraose acetate phthalate; pdb.
The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.
Four types of media containing 2% granulated agar (Difco, Detroit, MI) were used to grow fungi: (1) potato dextrose agar [ 31 ] , (2) potato sucrose agar (similar to potato dextrose agar except sucrose is used instead of dextrose; [ 31 ] , (3) casein agar (for 1 L, 5 g casein, 0.5 g NaCl, 20 g sucrose, and 20 g agar), and (4) sagebrush-leaf agar.
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