Words similar to naci

Example sentences for: naci

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Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.
LH was extracted from the cells [ 36 ] by removing and discarding the medium and adding 1 ml extraction buffer (150 mM NaCI, 50 mM Tris, 5 mM EDTA, and 1 mM bacitracin, pH 7.4) followed by freeze-thawing twice.
Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).
4, 135 mM NaCI, 4.5 mM KCL, 1.5 mM CaCl 2 , 0.5 mM MgCL 2 , 1.0 mM NaH 2 PO4, 5.6 mM glucose, 0.3% BSA, and 10 -9M estradiol) containing 5 μCi of [ 3H]-myoinositol/ml of medium.
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).