Words similar to naci

Example sentences for: naci

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5, NaCI 150 mM, Triton X-100 0.10%, glycerol 10%) followed by an additional washing with HNTG buffer + 1% bovine serum albumin (BSA).
Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.
4, 135 mM NaCI, 4.5 mM KCL, 1.5 mM CaCl 2 , 0.5 mM MgCL 2 , 1.0 mM NaH 2 PO4, 5.6 mM glucose, 0.3% BSA, and 10 -9M estradiol) containing 5 μCi of [ 3H]-myoinositol/ml of medium.
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.
LH was extracted from the cells [ 36 ] by removing and discarding the medium and adding 1 ml extraction buffer (150 mM NaCI, 50 mM Tris, 5 mM EDTA, and 1 mM bacitracin, pH 7.4) followed by freeze-thawing twice.