Words similar to naci

Example sentences for: naci

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LH was extracted from the cells [ 36 ] by removing and discarding the medium and adding 1 ml extraction buffer (150 mM NaCI, 50 mM Tris, 5 mM EDTA, and 1 mM bacitracin, pH 7.4) followed by freeze-thawing twice.
Cells (P-100 plates) were switched to physiological salt solution (4 ml) [consisting of (in mmol/L) NaCI, 103; KCI, 4.7; KH 2 PO 4 , 1.18; MgSO 4 • 7H 2 O, 1.17; CaCl 2 • 2H 2 O, 1.6; NaHCO 3 , 14.
4, 135 mM NaCI, 4.5 mM KCL, 1.5 mM CaCl 2 , 0.5 mM MgCL 2 , 1.0 mM NaH 2 PO4, 5.6 mM glucose, 0.3% BSA, and 10 -9M estradiol) containing 5 μCi of [ 3H]-myoinositol/ml of medium.
Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.