Words similar to naci
Example sentences for: naci
How can you use “naci” in a sentence? Here are some example sentences to help you improve your vocabulary:
0 gm/L NaCI, 380 mg/L KCl, 100 mg/L Na 2 HPO 4 , 3.0 gm/L Tris HCI, 200 mg/L CaCl 2 · 2H 2 O, 100 mg/L MgCl 2 · 6H 2 O; pH 7.4).
Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.
Plates were washed with ice-cold phosphate-buffered saline containing sodium orthovanadate as a tyrosine phosphatase inhibitor (10 mM sodium phosphate, 150 mM NaCI, 1 mM Na 3 VO 4 , pH 7.0).
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
Forty μg of gp120 were mixed with 40 μg of CAP in 1 ml TS or 1 M NaCI, 0.01 M Tris, pH 7.0 and incubated at 20°C for 30 min.