Words similar to naci

Example sentences for: naci

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Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.
Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).
They were then lysed in a protein kinase lysis buffer [50 mM HEPES, pH 7.5, 150 mM NaCI, 1.5 mM MgCI2, 1 mM EGTA, 1 mM sodium vanadate, 50 mM sodium fluoride, 20 mM beta-glycerophosphate, 10% (vol/vol) glycerol, 1% (vol/vol) Triton X-100, and protease inhibitors (1 mM PMSF, 1 ug/ml pepstatin A1, 1 ug/ml leupeptin, and 1 ug/ml aprotinin].
Cells (P-100 plates) were switched to physiological salt solution (4 ml) [consisting of (in mmol/L) NaCI, 103; KCI, 4.7; KH 2 PO 4 , 1.18; MgSO 4 • 7H 2 O, 1.17; CaCl 2 • 2H 2 O, 1.6; NaHCO 3 , 14.
0 gm/L NaCI, 380 mg/L KCl, 100 mg/L Na 2 HPO 4 , 3.0 gm/L Tris HCI, 200 mg/L CaCl 2 · 2H 2 O, 100 mg/L MgCl 2 · 6H 2 O; pH 7.4).