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Example sentences for: mhcii

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Another reported mechanism of IFNg resistance in tumor cells is associated with down-regulation of IFNg-receptors [ 38 ] . The relevance of this mechanism to MHCII inducibility was recently supported by the evidence that PMA is able to enhance IFNg-dependent MHCII expression in THP-1 human leukemia cells through the up-regulation of IFNg receptors [ 19 ] . In our experiments, however, the incubation of LS1034 cells with PMA and ethanol did not lead to any changes in IFNgR expression as determined by flow cytometry (Figure 4).
MSTO-211H, mesothelioma, cell line also showed a weak induction of MHCII in response to IFNg and HepG2, hepatocellular carcinoma, was completely non-inducible (data not shown).
In this study we showed that IFNg-inducibility of MHCII antigens in weakly inducible LS1034 colorectal carcinoma cell line can be rescued by concomitant incubation with PKC agonists.
An experiment performed to assess contribution of each of the three sources demonstrated that: (1) non-specific binding of IgG2a-FITC was negligible in all experimental groups (Fig 8H); (2) tumor cells incubated with PMA alone did not bind anti-MHCII mAb above the level of isotype control and (3) tumor cells incubated with PMA (or with PMA+IFNg) demonstrated 1.2-1.
We also show that the IFNg-dependent MHCII expression in LS1034 cell line can be rescued by clinically acceptable concentrations of Bryostatin 1.