Example sentences for: mgso
How can you use “mgso” in a sentence? Here are some example sentences to help you improve your vocabulary:
The diagnosis of each patient is shown in Table 1. Tissue samples were homogenized in a Dounce homogenizer on ice in 1 ml of lysis buffer (25 mM MES, 1 mM MgSO 4 , 2 mM EGTA, 0.1 mM GTP, 0.1% Triton X-100 with a protease inhibitor cocktail [Complete Protease Inhibitor Tablets; Boehringer Mannheim]).
Right-handed fibers were produced by overnight growth in 10ml TB containing 50 mM MgSO 4 at 20°C.
Labeled cells were incubated in 1 ml HEPES-buffered saline solution (HBS, composition in mM: NaCl, 145; KCl, 5; MgSO 4 , 1; CaCl 2 , 1.8; glucose, 10; HEPES, 10; pH 7.4) at 37°C for 30 min twice to wash out the unincorporated label.
MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.
5, 100 mM NaCl, 1 mM EGTA, and 4 mM MgSO 4 . Cells were then resuspended in 0.6 ml of the same buffer plus 1% β-mercaptoethanol, 0.5 mM phenyl methyl sulfonyl fluoride, 50 μg/ml leupeptin, 4 μg/ml aprotinin, and 15 μl/ml of protease inhibitor cocktail P8215 (Sigma).
Loading...