Words similar to mgso

Example sentences for: mgso

How can you use “mgso” in a sentence? Here are some example sentences to help you improve your vocabulary:

Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).
To study the effect of pH on the fluorescence of oxidized OxyBURST Green H 2 HFF BSA and 5-(and -6)-carboxyfluorescein, 78 μg/mL of the oxidized dye or 4.2 μM 5-(and -6)-carboxyfluorescein were prepared in MES (4-morpholineethanesulfonic acid) buffer solutions containing 5 mM NaCl, 115 mM KCl, 1.2 mM MgSO 4 and 25 mM MES (pHs from 4.5 to 7.5).
Slices were allowed to incubate and recover for at least an hour in room temperature ACSF comprised of the following composition (mM): NaCl 117; KCl 5.4; NaHCO 3 26; MgSO 4 1.3; NaH 2 PO 4 1.2; CaCl 2 2.5; glucose 10, continuously bubbled with 95% O 2 plus 5% CO 2 .
4 mM of each dNTP, 2.4 mM MgSO 4 ), 1 μl of each primer (0.
Specifically, 5 μl of the original 100 μl reaction was transferred to new plates and mixed with 95 μl reaction mix 2 (For 110 reactions; 1.1 ml 10X Buffer, 220 μl 50 mM MgSO 4 , 165 μl premixed 10 mM dNTP's, 550 μl 20 μM universal primer pair, 1.1 ml Pfx Enhancer reagent, 88 μl Pfx enzyme, and 7.227 ml sterile, deionized water).