Words similar to mgcl

Example sentences for: mgcl

How can you use “mgcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.
Top67 and the ZD domains at different concentrations were mixed and incubated at 37°C for 10 min before addition to the 0.3 μg of supercoiled plasmid DNA in 20 μl of 10 mM Tris-HCl pH 8.0, 2 mM MgCl 2 , 0.1 mg/ml gelatin.
0 MΩ were filled with filtered intracellular solution containing 145 mM KCl, 2 mM MgCl 2 , 1 mM EGTA, 25 mM HEPES (pH 7.4).
Amplification reactions were performed in a total volume of 40 μl and contained 2 μl eluted phage, 40 ng of each primer (FADI 5'-CACTACAATGGATGATG-3' and RADI 5'-CTTGCGGGGTTTTTCAG-3'), 0.001% Tween 20 (Sigma), 2.5 U Taq DNA polymerase (Promega), 1x Taq polymerase buffer (Promega), 1.56 mM MgCl 2 (Promega), and 0.25 mM of each dNTP (USB).
5 mM MgCl.