Words similar to mgcl

Example sentences for: mgcl

How can you use “mgcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.
Full-length ds-cDNA was synthesized by adding 57 μl nuclease-free water, 10 μl 10× PCR buffer (Roche), 10 μl 25 mM MgCl 2 , 1 μl 10 mM dNTP and 5 u Ampli-Taq Gold DNA Polymerase (Roche).
For Mg 2+titration, 0 mM, 1.5 mM, 2.5 mM, 3.5 mM and 4.5 mM MgCl 2 were included in each reaction.
2/ 10 mM MgCl 2 / 0.2 mM EDTA/ 5 μM ATP/10 μCi 32P-ATP) in a final volume of 40 μl.