Example sentences for: mgcl

How can you use “mgcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After incubation on ice for 12 min, MgCl 2 was added to a final concentration of 3 mM and incubation continued for an additional 5 min.

  • PCR amplifications were carried out in a 4 μl volume containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 0.001% gelatin, 100 μM each dNTP, 0.075 μM of fluorescent-labelled forward and unlabeled reverse primer, and a 0.12 units of Taq polymerase.

  • 5 MΩ) were filled with intracellular recording buffer containing 25 mM HEPES pH 7.4, 145 mM KCL, 2 mM MgCl 2 and 1 mM EGTA.

  • 9, 10 mM KCl, 2 mM MgCl 2 , 0.1 mM EDTA, 1 mM DTT, and 1 mM PMSF) before lysis with NP40.

  • As a result, Top67 could position itself in the absence of ZD domain at the junction of double- and single-stranded DNA for subsequent DNA cleavage, as previously observed for intact topoisomerase I [ 8 ] . Reversal of DNA cleavage also took place readily with Top67 upon addition of 1 M NaCl and 4 mM MgCl 2 . The ZD domain also was not required for selectivity of a cytosine in the -4 position relative to the cleavage sites.


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