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Example sentences for: mgcl

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The level of PR-1 induction shown by the double mutant in response to DC3000• avrRpt2 was not significantly different from that shown in response to either DC3000•empty vector or the MgCl 2 blank (Student's t test, P > 0.05).
0 gm/L NaCI, 380 mg/L KCl, 100 mg/L Na 2 HPO 4 , 3.0 gm/L Tris HCI, 200 mg/L CaCl 2 · 2H 2 O, 100 mg/L MgCl 2 · 6H 2 O; pH 7.4).
Incorporation of tritiated lysine into tRNA was measured by modification of the protocol of Hou [ 51 ] . Reactions contained 10 micrograms of total protein in 20 mM KCl, 10 mM MgCl 2 , 4 mM dithiothreitol, 2 mM ATP, 50 mM Na HEPES pH 7.5, 20 μM lysine (10 μCi 3H lysine), and were performed with or without 15 μg yeast tRNA (Sigma) in a total volume of 60 μl.
3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.