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Example sentences for: mgcl

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For the assay, 30-40 μl of this supernatant was used in a 3.1 ml assay mixture that contained: 1.5 mM MgCl 2 , 26 mM histidine solution (pH 7.6), 8 μM glucose-1,6-diphosphate, 0.45 U/ml glucose-6-phosphate dehydrogenase, and 0.2 mM NADP.
Nuclei were prepared from HeLa cells by standard procedures [ 35] and resuspended in 10 packed nuclei volumes of buffer A (10 mM Hepes (pH 7.9) 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 0.5 mM PMSF).
The primers were 5'-labeled using γ- 32P-ATP and T4 polynucleotide kinase according to the standard protocol [ 37 ] . Polymerase reactions were carried out by incubating 2.5 nM template primer with 50 nM of the wild type HIV-1RT or its mutant derivative in a total reaction volume of 6 μL containing 25 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 100 μg/mL bovine serum albumin, 5 mM MgCl
The bath and control perfusion solutions were the same and contained (in mM): 100 NaCl, 2 KCl, 1 MgCl 2 , 1 CaCl 2 , 11 glucose, 10 HEPES at pH 7.4.
Lung neutrophil cDNA was then amplified in 20 μl buffer containing 1 μl neutrophil reverse transcribed cDNA (representing 10 ng total RNA), 10 mM Tris-HCl pH 8.3, 50 mM KCl, 1.5 mM MgCl