Example sentences for: mfold

How can you use “mfold” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Since mfold tends to insert small helices within terminal loops that falsely reduce the size of the terminal loop, small terminal loops (3, 4 or 5 nucleotides in length) were extended to seven nucleotides.

  • All mfold outputs were parsed, and information about the number of structures, number of arms per structure, size of helices within arms and size and symmetry of the internal loops within arms was loaded into the MySQL database.

  • The mfold computer program version 3.0 http://bioinfo.math.rpi.edu/~mfold/rna/form1.cgiwas used to predict the RNA secondary structure and to calculate the folding free energy [ 26 27 ] . We have computed the minimum-free-energy structures from all the donor- and acceptor sites of the 60 exons of NF1 within a window of 100 bp.

  • Given a 100-nucleotide input sequence, mfold 3.1 typically returns one to six alternate structures, each containing one to four helical arms; thus, the structure containing the highest-scoring helical arm had to be determined for each folded sequence.

  • We analyzed conserved regions with mfold 3.1, an RNA-folding algorithm [ 41 ] . As miRNA genes can be located on either strand, and the quality of predicted hairpin structures can vary significantly between the respective strands (depending on the amount of G-U base-pairing), we folded each region as both the forward and the reverse complement of the extracted sequence (436,000 regions × 2 = 872,000 mfolds).


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