Words similar to mek
Example sentences for: mek
How can you use “mek” in a sentence? Here are some example sentences to help you improve your vocabulary:
Treatment of MDCK cells with 30 μM PD098059, a less potent inhibitor of MEK [ 28 29 ] , resulted in a ~50% reduction in AA release in response to 100 μM ATP, 1 μM IONO, and 10 μM IONO (data not shown).
In addition, using cells expressing wild-type cPLA 2 and cPLA 2 with S505A or S727A mutations, it was found that translocation kinetics and membrane targeting in response to ATP or ionomycin was similar to wild-type cPLA 2 . These data suggest that MEK1 inhibition reduces cPLA 2 catalytic activity and AA release independently of phosphorylation and translocation.
In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .
Due to the increased molecular weight of the EGFP-tagged cPLA 2 , the two forms of cPLA 2 did not separate as well, making the gel shift more difficult to visualize, but generally mirrored the gel shift characteristics of the endogenous cPLA 2 . These results suggest that, in response to MEK1 inhibition, there is a quantitative, dose-dependent decrease in AA release that correlates well with the loss of ERK activation, but not with the extent of cPLA 2 Ser 505phosphorylation.
Effect of MEK inhibition on AA release, ERK activation, and cPLA 2Ser 505phosphorylation
Loading...