Words similar to megascript
Example sentences for: megascript
How can you use “megascript” in a sentence? Here are some example sentences to help you improve your vocabulary:
A megascript T7 transcription kit (Ambion) was used to generate both sense and antisense strand RNA.
In vitro transcription of ds cDNA was performed using a T7 MEGAscript™ kit (Ambion ®).
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
The first IVT was performed with Megascript T7 (Ambion) in a 20 μl volume for 5-6 h, followed by DNA digestion.
Labeled cRNA was prepared with the MEGAscript In Vitro Transcription kit (Ambion, Inc., Austin, TX).