Words similar to megascript
Example sentences for: megascript
How can you use “megascript” in a sentence? Here are some example sentences to help you improve your vocabulary:
To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).
The in vitro transcription was performed with the T7 Megascript Kit (Ambion) per manufacturer's instructions, except that the 37°C incubation was increased to 16 hours.
In vitro transcription of ds cDNA was performed using a T7 MEGAscript™ kit (Ambion ®).
The first IVT was performed with Megascript T7 (Ambion) in a 20 μl volume for 5-6 h, followed by DNA digestion.
A megascript T7 transcription kit (Ambion) was used to generate both sense and antisense strand RNA.