Words similar to megascript

Example sentences for: megascript

How can you use “megascript” in a sentence? Here are some example sentences to help you improve your vocabulary:

The in vitro transcription was performed with the T7 Megascript Kit (Ambion) per manufacturer's instructions, except that the 37°C incubation was increased to 16 hours.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
In vitro transcription of ds cDNA was performed using a T7 MEGAscript™ kit (Ambion ®).
Labeled cRNA was prepared with the MEGAscript In Vitro Transcription kit (Ambion, Inc., Austin, TX).
The anti-sense RNA (aRNA) amplification by T7 in vitro transcription was carried out using MEGAscript T7 Kit (Ambion Inc., Austin, TX) following the manufacturer's instructions.