Example sentences for: megascript

How can you use “megascript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pDRF and pDXB were used for the production of internally 32P -labeled RNA substrates by in vitro transcription with the appropriate Megascript kit (Ambion) with addition of 20 μCi [α 32P]UTP (Amersham).

  • Labeled cRNA was prepared with the MEGAscript In Vitro Transcription kit (Ambion, Inc., Austin, TX).

  • To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).

  • The anti-sense RNA (aRNA) amplification by T7 in vitro transcription was carried out using MEGAscript T7 Kit (Ambion Inc., Austin, TX) following the manufacturer's instructions.

  • The in vitro transcription was performed with the T7 Megascript Kit (Ambion) per manufacturer's instructions, except that the 37°C incubation was increased to 16 hours.


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