Words similar to megascript

Example sentences for: megascript

How can you use “megascript” in a sentence? Here are some example sentences to help you improve your vocabulary:

pDRF and pDXB were used for the production of internally 32P -labeled RNA substrates by in vitro transcription with the appropriate Megascript kit (Ambion) with addition of 20 μCi [α 32P]UTP (Amersham).
The first IVT was performed with Megascript T7 (Ambion) in a 20 μl volume for 5-6 h, followed by DNA digestion.
The anti-sense RNA (aRNA) amplification by T7 in vitro transcription was carried out using MEGAscript T7 Kit (Ambion Inc., Austin, TX) following the manufacturer's instructions.
The in vitro transcription was performed with the T7 Megascript Kit (Ambion) per manufacturer's instructions, except that the 37°C incubation was increased to 16 hours.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).