Example sentences for: mcs

How can you use “mcs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resulting plasmid was called pBigT, and its MCS contains sites for the restriction enzymes NheI, SalI, AccI, XhoI, ApaI, SacII, NotI, SacI and BclI.

  • The medium was changed every other day, the identity of the VSMCs was confirmed by α-actin fluorescence staining as described [ 45 ] . Glomerular MCs, CCD cells and MICs were cultured as we previously reported [ 22 44 46 ] .

  • The resulting plasmid was digested with XbaI and ApaI to remove most of the 3' MCS (except for the terminal KpnI site), which was replaced with an AscI linker.

  • The resulting plasmid was digested with SacI and PstI to remove the 5' MCS, which was replaced by a PacI linker.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.


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