Example sentences for: mcs

How can you use “mcs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resulting plasmid was digested with XbaI and ApaI to remove most of the 3' MCS (except for the terminal KpnI site), which was replaced with an AscI linker.

  • The resulting plasmid was digested with SacI and PstI to remove the 5' MCS, which was replaced by a PacI linker.

  • The bpA sequence along with the 3' AscI site was excised from pbpAAsc by digesting it with SacI and KpnI, and inserted downstream of the MCS in pSAleotpA by digesting it with SacI and KpnI.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.

  • The medium was changed every other day, the identity of the VSMCs was confirmed by α-actin fluorescence staining as described [ 45 ] . Glomerular MCs, CCD cells and MICs were cultured as we previously reported [ 22 44 46 ] .


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