Words similar to lipofectin
Example sentences for: lipofectin
How can you use “lipofectin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cos-7 and NIH3T3 cells were grown in DMEM containing 10% FCS and antibiotics at 37°C with 5% CO 2 . Cos-7 cells were transfected by calcium phosphate method, while the NIH3T3 cells were transfected using Lipofectin (Gibco, BRL).
The 1.2 kb coding sequence of the receptor was subcloned into pCI-neo ®mammalian expression vector (Promega, Madison, WI) using the restriction sites EcoRI and Sal I. HEK 293 cell were transfected with the vector using the lipofectin ®reagent (Life Technologies, Rockville, MD).
This finding underscores the versatility of PEI as a method for introducing genes into postmitotic populations of cells, particularly as our previous efforts to transfect sympathetic neurons using lipid-based reagents (Lipofectamine and Lipofectin) had been unsuccessful.
A solution (1 ml) of DMEM containing the oligonucleotides at the indicated concentration and 15 μg/ml DOTMA/DOPE (Lipofectin ®; GIBCO-BRL) was then added.
Tissue culture medium, Lipofectin ®and TRIzol ®were purchased from Life Technologies (Gaithersburg, MD).