Example sentences for: lipofectamine

How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Transfections were performed in 6-well plates or 6 cm culture dishes using Lipofectamine (GibcoBRL) or FuGene6 (Roche) according to the manufacturer's protocol.

  • The SN56 cells were grown to about 50-70% confluence, then transiently transfected with these plasmids using Lipofectamine Plus reagent according to the manufacturer's instructions.

  • A549 cells were grown to 50-80% confluence on 12-well plates and transfected with the Mercury pathway profiling system vector, pNFκB-TA-Luc (Clontech, Palo Alto, CA, USA), using lipofectamine (Gibco/BRL, Rockville, MD, USA) and used according to the manufacturer's recommendations.

  • The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.

  • The oligonucleotides were diluted in 100 μl Opti-MEM to various concentrations, mixed with 100 μl of Opti-MEM containing 6 μl of Lipofectamine and incubated at room temperature for 20 minutes.


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