Example sentences for: lipofectamine

How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The SN56 cells were grown to about 50-70% confluence, then transiently transfected with these plasmids using Lipofectamine Plus reagent according to the manufacturer's instructions.

  • Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and Lipofectamine Plus reagents were from Gibco BRL Life Technologies (Grand Island, NY).

  • 2 ug of plasmid DNA and 25 ul of Lipofectamine were combined in 200 ul of Opti-mem media (Life Technologies) and allowed to stand at room temperature for 30 minutes.

  • DNA/lipofectamine complexes were allowed to form for 30 min at room temperature in RPMI (without antibiotics) containing 1 μg of reporter plasmid, 20 ng of pVAX-LacZ for monitoring transfection efficiency, and 10 μg of lipofectamine.

  • The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.


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