Example sentences for: lipofectamine

How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DNA/lipofectamine complexes were allowed to form for 30 min at room temperature in RPMI (without antibiotics) containing 1 μg of reporter plasmid, 20 ng of pVAX-LacZ for monitoring transfection efficiency, and 10 μg of lipofectamine.

  • Transfections were performed in 6-well plates or 6 cm culture dishes using Lipofectamine (GibcoBRL) or FuGene6 (Roche) according to the manufacturer's protocol.

  • YZ4 was then subcloned into the eukaryotic expression vector pcDNA3 (Invitrogen) and the resultant plasmid, pcDNA3/REP 2 , was transfected into COS-1 using lipofectamine according to the manufacturer's directions (Life Science Technologies) with 12 μg plasmid DNA and 45 μL lipofectamine solution.

  • Following linearization with Bgl II, the constructs were transfected into the P19 cell line using Lipofectamine (Gibco-BRL) at a concentration of 25 μl per 100 ml of serum free medium.


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