Words similar to lipofectamine
Example sentences for: lipofectamine
How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:
Synthetic vectors include cationic polymers such as polyethyleneimine (PEI) and polylysine, as well as cationic lipids such as Lipofectamine [ 2] and negatively charged liposomes [ 3].
The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.
A549 cells were grown to 50-80% confluence on 12-well plates and transfected with the Mercury pathway profiling system vector, pNFκB-TA-Luc (Clontech, Palo Alto, CA, USA), using lipofectamine (Gibco/BRL, Rockville, MD, USA) and used according to the manufacturer's recommendations.
DNA/lipofectamine complexes were allowed to form for 30 min at room temperature in RPMI (without antibiotics) containing 1 μg of reporter plasmid, 20 ng of pVAX-LacZ for monitoring transfection efficiency, and 10 μg of lipofectamine.
α s -GFP fusion proteins were assayed for the ability to stimulate adenylyl cyclase in response to luteinizing hormone (LH) receptor stimulation [ 38 ] . 10 6HEK 293 cells/60 mm-dish were co-transfected with 2 μg of plasmid DNA encoding the α s -GFP fusion protein, and 0.2 μg of plasmid DNA encoding the rat LH receptor in pCIS [ 39 ] , using 10 μL of Lipofectamine 2000.