Words similar to lipofectamine
Example sentences for: lipofectamine
How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:
The SN56 cells were grown to about 50-70% confluence, then transiently transfected with these plasmids using Lipofectamine Plus reagent according to the manufacturer's instructions.
Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and Lipofectamine Plus reagents were from Gibco BRL Life Technologies (Grand Island, NY).
2 ug of plasmid DNA and 25 ul of Lipofectamine were combined in 200 ul of Opti-mem media (Life Technologies) and allowed to stand at room temperature for 30 minutes.
DNA/lipofectamine complexes were allowed to form for 30 min at room temperature in RPMI (without antibiotics) containing 1 μg of reporter plasmid, 20 ng of pVAX-LacZ for monitoring transfection efficiency, and 10 μg of lipofectamine.
The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.