Example sentences for: lipofectamine

How can you use “lipofectamine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The oligonucleotides were diluted in 100 μl Opti-MEM to various concentrations, mixed with 100 μl of Opti-MEM containing 6 μl of Lipofectamine and incubated at room temperature for 20 minutes.

  • 2 ug of plasmid DNA and 25 ul of Lipofectamine were combined in 200 ul of Opti-mem media (Life Technologies) and allowed to stand at room temperature for 30 minutes.

  • The DNA was mixed with 2 μl of Lipofectamine 2000 reagent (Life Technologies, Rockville, MD, USA) in a total volume of 50 μl of serum-free RPMI per cm 2of cells, incubated for 3 hours, and followed by the addition of an equal volume of media containing 20% fetal calf serum.

  • The finding that IL-1β expression and MAPK activation are concomitantly increased by CSC exposure and inhibited by PD98059 is consistent with the report that MAPKs activate expression of IL-1β genes [ 10 ] . Although the control of cytok-80% confluence on 12-well plates and transfected with the Mercury pathway profiling system vector, pNFκB-TA-Luc (Clontech, Palo Alto, CA, USA), using lipofectamine (Gibco/BRL, Rockville, MD, USA) and used according to the manufacturer's recommendations.

  • Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and Lipofectamine Plus reagents were from Gibco BRL Life Technologies (Grand Island, NY).


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