Words similar to linearized
Example sentences for: linearized
How can you use “linearized” in a sentence? Here are some example sentences to help you improve your vocabulary:
Constructs in this vector were linearized at appropriate restriction sites to allow the sythesis of digoxigenin-UTP labeled complementary RNA in the antisense or sense orientation (using T3 or T7 RNA polymerase, respectively; Boehringer, Indianapolis, IN).
This example illustrates how a robustness analysis of the linearized system can be used to deduce the robustness of the original non-linear system, as it shows that when the linearized system is unstable, the desired behaviour of the non-linear system will no longer be present.
Plasmids containing the ribozyme or the substrate (either 45 bp of M6P/IGF2R mRNA or an unmatched sequence 5'-ATATTGTCCAGTGC-3') were linearized with Mlu I. All transcripts were generated with T7 RNA polymerase (Promega).
This plasmid was subsequently linearized with KpnI and used for electroporation.
pURA4- rec12-Y98F was linearized by digestion with Nco I. Transformation, forward selection for Uracil prototrophy, and reverse selection for FOA resistance were as described [ 61 63 ] . Candidates were screened as described above.
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