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Example sentences for: linears

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The other recombination-defective mutants, in otherwise rec +background, did not accumulate the huge linears probably because the DNA was degraded by active RecBCD enzyme or was not produced.
In the recBC sbcBC strain, RecBCD enzyme is inactive and RecFOR and RecQJ proteins promote recombination together with RecA [ 19 ] . In the recBC sbcBC background, recA, recF, recJ, recQ and recR mutants accumulated these huge linears to varying extents (Figure 2D).
The other non-null recBCD mutants ( recC1001, recC1002, recC1003, recC1004, recC2145, recB2154 , and recB2155 ) are all nuclease positive [ 21 22 ] . They would be expected to degrade the huge linears.
One might expect that the accumulation of the huge linears may correlate with the capacity for recombination repair that reconstitutes a circular form.
In the recBC sbcA strain, an sbcA mutation on the Rac prophage expresses recET genes, which promotes homologous recombination at a ds end [ 7 ] . The accumulation of the huge linears was seen with recA, recE, recT, recJ, recQ, recF, recO and recR strains (Figure 2C).