Words similar to lcrv
Example sentences for: lcrv
How can you use “lcrv” in a sentence? Here are some example sentences to help you improve your vocabulary:
All of the alanine mutants screened were capable of interaction with LcrV as demonstrated in a filter-lift assay.
Our subsequent studies suggest that the interaction of LcrG with LcrV occurs at a hydrophobic patch within LcrG.
Results presented in this study demonstrate that the smallest linear region of LcrG required for interaction with LcrV is residues 7 to 40 (highlighted in yellow in Fig.
To further understand the phenotypes obtained, the ability of LcrG S23R and L30R to interact with LcrV was examined in Y. pestis . Wild type LcrG, LcrG S23R, LcrG L30R, and LcrG A16R were independently co-expressed with His 6 -tagged LcrV in a strain of Y. pestis deleted for lcrGV . The cells were French pressed and the cleared lysates were applied to Talon columns to purify His 6 -tagged LcrV.
Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.