Words similar to lcrv
Example sentences for: lcrv
How can you use “lcrv” in a sentence? Here are some example sentences to help you improve your vocabulary:
PcrG does not complement an lcrGstrain, but interacts with LcrV
The weaker interaction seen in Y. pestis between LcrG S23R and LcrV corresponds with our yeast two-hybrid data for the S23R mutant, which showed an interaction with LcrV, and suggests an interaction with decreased affinity (Table 2).
All of the alanine mutants screened were capable of interaction with LcrV as demonstrated in a filter-lift assay.
Therefore, while PcrG is capable of interacting with LcrV, it differs from LcrG in its ability to block secretion of Yops in Y. pestis .
Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.