Words similar to lcrg
Example sentences for: lcrg
How can you use “lcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:
LcrG and LcrV physically interact in Y. pestis [ 4 ] , and that interaction plays a significant role in the regulation of Yops secretion [ 20 ] . LcrG and LcrV interact in a yeast two-hybrid assay [ 20 ] , therefore this technique was used to further investigate the region of LcrG required for its interaction with LcrV.
Mutagenesis of the serine at position 23 to arginine resulted in an LcrG variant that weakly interacted with LcrV as determined by a filter-lift assay for β-galactosidase activity.
Surprisingly, the alanine-scanning mutagenesis results failed to demonstrate amino acids of LcrG involved in the LcrG-LcrV interaction.
Analysis of the putative secretion-blocking region of LcrG
This result suggested that mutagenesis of pcrG could be used to isolate PcrG mutants capable of blocking Yops secretion in Y. pestis . This genetic screen allowed the isolation of PcrG mutants that functioned in Y. pestis in a manner indistinguishable from LcrG (Fig.