Example sentences for: lcrg

How can you use “lcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The LcrG-titration model theorizes that LcrG blocks the secretion apparatus (Ysc) from the bacterial cytoplasm and that interaction with LcrV is required to relieve the blockage.

  • Upon induction by eukaryotic cell contact or removal of calcium from the growth medium, the intracellular level of LcrV becomes elevated relative to that of LcrG.

  • This result suggested that mutagenesis of pcrG could be used to isolate PcrG mutants capable of blocking Yops secretion in Y. pestis . This genetic screen allowed the isolation of PcrG mutants that functioned in Y. pestis in a manner indistinguishable from LcrG (Fig.

  • Transformants that grew at 37°C were streak purified and checked for phenotype by determining Ca 2+-dependence, which suggests restoration of "LcrG function" by PcrG.

  • Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.


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