Words similar to lcrg
Example sentences for: lcrg
How can you use “lcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:
The LcrG-titration model theorizes that LcrG blocks the secretion apparatus (Ysc) from the bacterial cytoplasm and that interaction with LcrV is required to relieve the blockage.
Upon induction by eukaryotic cell contact or removal of calcium from the growth medium, the intracellular level of LcrV becomes elevated relative to that of LcrG.
This result suggested that mutagenesis of pcrG could be used to isolate PcrG mutants capable of blocking Yops secretion in Y. pestis . This genetic screen allowed the isolation of PcrG mutants that functioned in Y. pestis in a manner indistinguishable from LcrG (Fig.
Transformants that grew at 37°C were streak purified and checked for phenotype by determining Ca 2+-dependence, which suggests restoration of "LcrG function" by PcrG.
Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.