Words similar to kc

Example sentences for: kc

How can you use “kc” in a sentence? Here are some example sentences to help you improve your vocabulary:

Polymerase chain reaction (PCR) was carried out in an 11-μl reaction volume containing 0.5 pM of each primer, 0.2 mM dNTP, 2 mM MgCl 2 , 5% glycerol, 0.275 U Taq polymerase, 50 mM KC1, 10 mM Tris-HCl pH 8.3, and 0.1 μg of genomic DNA.
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
6 mM KC1, 1.5 mM MgCh, 5 mM DTT], 20 μM of each deoxribonucleoside triphosphate (dNTP), and 0.2 μM of one of the following anchored primers: H-T 11 -A, H-T 11 -G, or H-T 11 -C (H= AAGCTT).
Each PCR included 2 μl of the RT reaction containing the primary strand cDNA, reaction buffer [10 mM Tris-HCl (pH 8.4), 50 mM KC1, 1.5 mM MgCl 2 , 0.001% gelatin], 2 μM of each dNTP, 0.2 μM of the same anchored primer used in the RT reaction, 0.2 μM of one of the eight 13-mer arbitrary primers API-8 (Table 1), 12.
Two hundred ng of rat lung total RNA was added to a reaction which contained 10 mM Tris-HCl (pH 8.3), 50 mM KC1, 1.5 mM MgCl