Example sentences for: ivt
How can you use “ivt” in a sentence? Here are some example sentences to help you improve your vocabulary:
While analyzing the size distribution of the IVT product we noted a low molecular weight species that roughly corresponds in size to the primer adapter.
Next, we assessed the fidelity of amplification by comparing the IVT and R-PCR datasets with the direct labeling dataset.
IVT proteins were incubated with the GST fusion proteins on beads for 1 hour at 4°C in 500 μL of 40 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 1% Triton X100, 1 mM ATP, 1 μM microcystin, 14 mM b-mercaptoethanol.
These plots also illustrate another difference between the amplification protocols: an examination of best fit lines calculated for each of the plots suggests that dynamic range is increased by IVT amplification (slope > 1), but decreased by R-PCR amplification (slope < 1).
The IVT product retains essentially the same size distribution as the starting material.
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