Example sentences for: ivt

How can you use “ivt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These differences include: increased precision in spiked transcript concentration through dilution from the same IVT product; utilization of Test2 chips reflecting current probe sets and production chemistry; no post-hybridization streptavidin amplification; multiple measurements per condition; comparison of transcript intensities obtained with absent or present complex cRNA background; and the use of commercially available Test2 arrays rather than custom-made arrays containing hundreds of probe pairs per gene.

  • The high correlation between the IVT and direct labeling datasets is evident in the scatter plot shown in Fig 4. An analogous plot portrays the lower correlation between the R-PCR and direct labeling datasets.

  • The cDNA was purified using a Qiagen QIAquick purification kit, dried down, and resuspended in IVT reaction mix, containing 3.0 μl nuclease-free water, 4.0 μl 10X reaction buffer, 4.0 μl 75 mM ATP, 4.0 μl 75 mM GTP 3.0 μl 75 mM CTP, 3.0 μl 75 mM UTP, 7.5 μl 10 mM Biotin-11-CTP, 7.5 μl 10 mM Biotin 11-UTP and 4.0 μl enzyme mix (unmodified control condition).

  • For phosphatase treatment, IVT proteins were first incubated in 130 μl of binding buffer containing 20 U of alkaline phosphatase in the presence or absence of 10 mM Na 3 VO 4 for 1 h at 37°C.

  • IVT protocols are reportedly sensitive to excess starting concentrations of the T7 primer.

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