Example sentences for: ivt
How can you use “ivt” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first IVT was performed with Megascript T7 (Ambion) in a 20 μl volume for 5-6 h, followed by DNA digestion.
Consistent with the correlation analysis, the individual replicates for the IVT and direct labeling protocol cluster together, while the R-PCR replicates cluster in a separate arm of the dendrogram.
The high correlation between the IVT and direct labeling datasets is evident in the scatter plot shown in Fig 4. An analogous plot portrays the lower correlation between the R-PCR and direct labeling datasets.
Recently, studies employing DNA arrays have also examined the use of modified nucleotides such as DAP, 5-bromodeoxyuridine, and 2'-O-methylthymidine in the probe [ 21 22 ] . An alternative method to increase the probe-target affinity is to incorporate the modified ribonucleotide, as the triphosphate derivative, during the IVT process so that the cRNA produced has the desired level of substitution of the corresponding unmodified nucleotide.
A second advantage is that the IVT protocol maintains the size distribution of the starting material more effectively than R-PCR (Fig 2).
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