Example sentences for: ivm
How can you use “ivm” in a sentence? Here are some example sentences to help you improve your vocabulary:
M I oocytes were enucleated at 29 h after IVM and confluent or nocodazole-treated cells were transferred.
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.
Recently, a defined IVM system for porcine oocytes was developed in order to eliminate such variability [ 49, 50].
M II oocytes were enucleated at 42 h after IVM and embryos reconstituted with them were activated at 3 h after fusion.
Although this study did not compare defined IVM medium with complex medium the results indicate that protein-free IVM medium is suitable for maturing recipient oocytes for the production of cloned embryos.
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