Example sentences for: isotype-matched

How can you use “isotype-matched” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Staining for cell surface IFNg receptors was performed similarly, except that cells were first incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times and then stained with SA-Alf488.

  • In control experiments there was no immunostaining when isotype-matched control mouse IgG was used instead of the anti-CD68 antibody.

  • Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).

  • For each antibody, negative staining levels were set by comparison to an isotype-matched control.


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