Example sentences for: immunogold

How can you use “immunogold” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Initially, we used wild type (WT) tBid that was myc-tagged for immunogold electron microscopy in our investigation of its localization along the mitochondrial periphery.

  • To determine whether immunogold-labeled tBid is spatially clustered or randomly located about contact sites, a spatial point pattern statistics was used, namely, the nearest neighbor statistic [ 16 ] . The Euclidean distances from each immunogold particle to all of the contact sites in the reconstruction was calculated for all the particles using a similar approach to that described by Perkins and coworkers [ 13 ] . The nearest neighbor statistic was calculated for the reconstruction using the closest observed contact site to each gold particle and then calculating the mean and standard deviation for all such measurements in a reconstructed mitochondrion.

  • Before performing the time-expensive tomography, analysis of thin sections with conventional electron microscopy was used to judge the immunogold labeling quality.

  • Electron tomography has several advantages over conventional two-dimensional immunogold electron microscopy that should be enumerated to understand the accuracy of the measurements made in this study.

  • Although Rab24 has been proposed as a possible regulator of autophagic processes [ 24 43 ] , the accumulation of punctate bodies in cells expressing Rab24(D123I) was not prevented by 3-methyadenine, a known inhibitor of autophagosome formation [ 44 ] . Most importantly, electron microscopy with immunogold labeling of mycRab24(D123I) in ultra-thin sections confirmed the initial impression that the inclusions are in fact localized within the nucleus.


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