Example sentences for: immunofluorescence

How can you use “immunofluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Immunofluorescence microscopy indicates that α i2 (1-331) (Figure 4B) displays a subcellular localization pattern very similar to sα i2 (Figure 4A).

  • Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).

  • Cells were fixed and processed for immunofluorescence as described [ 4 20 ] . The N-terminal fragment expressed by fs-α 1S or wt-α 1S was identified with a mouse monoclonal antibody against a T7 epitope fused to the N-terminus of α 1S . The anti-T7 antibody (Novagen, WI) was used at a dilution of 1:1000.

  • The ongoing proliferation of HIV-1-infected podocytes following cell-cell contact led to the formation of multiple foci (Figure 2C) with intense, heterogenous nuclear cyclin D 1 immunofluorescence consistent with ongoing asynchronous proliferation in this population of cells (Figure 3).

  • The identity and purity of these cultures was established by immunofluorescent staining as previously described [ 9 ] (in addition to the extensive characterization by Clonetics, we have determined that these cells lack von Willebrand factor and platelet endothelial cell adhesion molecules (PECAM), but are positive for cytokeratin, immunofluorescence and alkaline phosphatase activity).


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