Example sentences for: immunofluorescence

How can you use “immunofluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In contrast, fixation with FAA, a mixture of formaldehyde, ethanol and acetic acid resulted in clear α-ARD immunofluorescence in neuropilar regions of the visual system (Fig.

  • As a comparison, a non-palmitoylated mutant of α q , in which cysteines 9 and 10 have been substituted with serines, is found predominantly in the soluble fraction (Figure 2E, lanes 7 and 8), as described previously [ 13 ] . Surprisingly, in comparison to α i2 , sα i2 was always detected in fewer cells, by immunofluorescence analysis, and showed lower levels of protein expression, by western blot analysis of subcellular fractions (Figure 2E) or whole cell lysates (not shown).

  • Immunofluorescence microscopy indicates that α i2 (1-331) (Figure 4B) displays a subcellular localization pattern very similar to sα i2 (Figure 4A).

  • Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).

  • We have previously demonstrated that these fusion proteins are kinase-active and exhibit localization patterns similar to those observed by indirect immunofluorescence for wild type Rlk . Furthermore, we have found that, like WT Rlk, they co-precipitate with Fyn (data not shown).


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