Words similar to immunofluorescence
- immunodetection
- immunodiagnostic
- immunoelectron
- immunoelectrophoresis
- immunoenzyme
- immunoexpression
- immunoflourescence
- immunoflourescent
- immunoflouresence
- immunofluorescence
- immunofluorescent
- immunofluoresence
- immunoflurescence
- immunogamma
- immunogen
- immunogenetics
- immunoglobulin
- immunoglobulins
- immunogold
Example sentences for: immunofluorescence
How can you use “immunofluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:
In S. cerevisiae , disassembly of the RENT complex and the subsequent release of Cdc14 from the nucleolus drive cells to exit mitosis [ 8 9 ] . This event is thought to require two independent signals: an unknown mitosis-specific signal and a signal from the mitotic exit network (MEN) proteins including Tem1, Cdc15, and Dbf2 [ 8 9 ] . The Polo-like kinase Cdc5 also belongs to the MEN, thus we tested whether it too is required for release of Cdc14 from the nucleolus by using indirect immunofluorescence to compare Cdc14 localization patterns in wild type and cdc5-1 cells.
Fixation with formaldehyde followed by extraction with Triton X-100 is a fairly standard method for immunofluorescence microscopy of cells, but microextensions were fragmented and sometimes even lost during this treatment as shown in Figure 2, A,B,C.
Briefly, this is an immunofluorescence assay in which 1:10 dilutions of the virus were prepared in shell vials and incubated at 37°C overnight.
The identity and purity of these cultures was established by immunofluorescent staining as previously described [ 9 ] (in addition to the extensive characterization by Clonetics, we have determined that these cells lack von Willebrand factor and platelet endothelial cell adhesion molecules (PECAM), but are positive for cytokeratin, immunofluorescence and alkaline phosphatase activity).
Furthermore, in sections where one of the antisera (either MIF or TH/SP) had been omitted, but still processed for double immunofluorescence, only the appropriate immunoreactivity was observed, indicating that the secondary antibodies were not cross reacting to produce spurious labeling (data not shown).
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