Words similar to hyclone
Example sentences for: hyclone
How can you use “hyclone” in a sentence? Here are some example sentences to help you improve your vocabulary:
SW-1353 cells (normal or stably transfected with pBkRSV-Bcl-3), human primary chondrocytes, or synovial fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA), penicillin/streptomycin and L-glutamine (Cellgro; Mediatech, Herndon, VA, USA) before use in experiments.
G418-resistant colonies from BRCA1 antisense infected BG-1 cells and control vector LXSN-infected BG-1 cells were pooled, pretreated for 5 days in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate for growth curve analysis in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3, 17β-diol; Sigma, St Louis, MO, USA).
The cells were then washed with PBS and cultured in RP10 (RPMI 1640(GIBCO/BRL)/10% FBS (HyClone)/0.
GTM66 cells were derived from a 92-year old woman with glaucoma and were cultured in low glucose DMEM (HyClone Laboratories) with 10% FBS (HyClone Laboratories) and antibiotics (Invitrogen) as described [ 11 17 ] . Cells were transfected with Lipofectamine 2000 reagent according to the manufacturers recommendations (Invitrogen).
2 × 105 per well) in 5% charcoal stripped serum (Hyclone, Logan, UT), antibiotics, and 2 mM L-glutamine in phenol red-free DMEM.