Example sentences for: hyclone

How can you use “hyclone” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The supernatant was carefully removed, and cells were collected by centrifugation and plated in DMEM containing 10% FBS (Hyclone, Logan, UT) for 48 hours.

  • Fetal bovine serum(Hyclone) 10% was used as supplement to the media (low glucose DMEM (GIBCO, BRL)) when indicated.

  • G418-resistant colonies from BRCA1 antisense infected BG-1 cells and control vector LXSN-infected BG-1 cells were pooled, pretreated for 5 days in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate for growth curve analysis in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3, 17β-diol; Sigma, St Louis, MO, USA).

  • GTM66 cells were derived from a 92-year old woman with glaucoma and were cultured in low glucose DMEM (HyClone Laboratories) with 10% FBS (HyClone Laboratories) and antibiotics (Invitrogen) as described [ 11 17 ] . Cells were transfected with Lipofectamine 2000 reagent according to the manufacturers recommendations (Invitrogen).

  • Clones were isolated from the BRCA1 antisense and LXSN BG-1 pooled populations and grown in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with charcoal/dextran treated serum (Hyclone) for 5 days before plating for growth curve analysis.


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