Words similar to hybond

Example sentences for: hybond

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Total RNA was size separated by electrophoresis on a 1.5% agarose/formaldehyde gel (10 μg of total RNA per lane) and transferred to a Hybond N+ nylon membrane.
Fractionated DNA in gels was depurinated, denatured, and neutralized according to standard protocols [ 76 ] . DNA was transferred to positively charged nylon membranes (Hybond N +) (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) by the wick transfer method using 20X SSC overnight.
25 μg) was directly transferred to Hybond N+ nylon membrane under vacuum using a Hoefer PR648 slot blot apparatus (Amersham Pharmacia Biotech) as described in [ 25 ] . The slot blots were produced in batches of ten from a relevant master mix and probed with the same cDNA under the same conditions as described for Northern blot analysis.
4 M formaldehyde RNA gel and transferred to Hybond N +nylon membrane (Amersham-Pharmacia, UK).
The gel was rinsed with diethylpyrocarbonate-treated H 2 O and the RNA was transferred to a Hybond N+ membrane (Amersham Biosciences) by capillary transfer in alkaline solution (0.