Words similar to hybond

Example sentences for: hybond

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Fractionated DNA in gels was depurinated, denatured, and neutralized according to standard protocols [ 76 ] . DNA was transferred to positively charged nylon membranes (Hybond N +) (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) by the wick transfer method using 20X SSC overnight.
The gel was rinsed with diethylpyrocarbonate-treated H 2 O and the RNA was transferred to a Hybond N+ membrane (Amersham Biosciences) by capillary transfer in alkaline solution (0.
Isolation of total cellular RNA using a urea/LiCl protocol and gel electrophoresis was performed as described previously [ 65 ] . Gels were transferred onto Amersham Hybond N+ nylon membranes in 20 × SSC and prehybridized in 4 × SSC, 1 × Denhardt's solution, 0.5% SDS for 2 h at 68°C.
4 M formaldehyde RNA gel and transferred to Hybond N +nylon membrane (Amersham-Pharmacia, UK).
Total RNA was size separated by electrophoresis on a 1.5% agarose/formaldehyde gel (10 μg of total RNA per lane) and transferred to a Hybond N+ nylon membrane.