Example sentences for: hybond-

How can you use “hybond-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The agarose gels were blotted to nylon membranes (Hybond-N+, Amersham, Arlington Heights, IL).

  • Total RNA from planarian (10 μg per line) were fractionated by 1,1% formaldehyde-agarose denaturing gel electrophoresis and transferred to the Hybond-N membrane that was used for hybridization with [α- 32P]-labeled probes.

  • Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'

  • Approximately 300 ng undigested planarian genomic DNA from each strain was resolved on agarose gels and transferred to Hybond-N membranes (Amersham).

  • Fractionated DNAs were transferred to Hybond-XL membrane (Amersham Pharmacia Biotech, Piscataway, NJ) as described by Sambrook et al.


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