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Example sentences for: hybond-

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The agarose gels were blotted to nylon membranes (Hybond-N+, Amersham, Arlington Heights, IL).
Approximately 300 ng undigested planarian genomic DNA from each strain was resolved on agarose gels and transferred to Hybond-N membranes (Amersham).
Total RNA from planarian (10 μg per line) were fractionated by 1,1% formaldehyde-agarose denaturing gel electrophoresis and transferred to the Hybond-N membrane that was used for hybridization with [α- 32P]-labeled probes.
Proteins were electroblotted to Hybond-P PVDF membranes (Amersham Pharmacia Biotech), blocked with gelatin, and probed with affinity purified rabbit anti-human myocilin antibody 129 (AB129; generated to human myocilin amino acids 156-171) [ 12 17 ] and an anti-rabbit IgG secondary antibody (Amersham).
Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'