Example sentences for: hybridoma

How can you use “hybridoma” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.

  • Additional primary antibodies used were zn-8 to detect Dmgrasp and zrf-1 (Developmental Studies Hybridoma Bank) and polyclonal rabbit anti Pax-2 (Berkeley Antibody Co.).

  • The plates were dried, immersed in 0.5% polyisobutylmethacrylate in acetone, and sequentially incubated with Stx-1, anti-Stx-1 monoclonal antibody (purified from a hybridoma cell line, 13C4 (ATCC, Rockville, MD), as previously described [ 9 ] ), and 125I-goat anti-mouse IgG (DuPont NEN, Boston, MA).

  • Our previous studies of the VH gene repertoire in RA SM [ 13] and studies using a hybridoma approach [ 8] revealed an over-representation of VH4 and a biased set of represented VH4 genes.

  • The sections were rinsed again and incubated overnight at 4°C with the Rip primary antibody (Developmental Studies Hybridoma Bank, Iowa City, IA; diluted 1:4 in .1 M PBS).


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