Words similar to hybridoma
Example sentences for: hybridoma
How can you use “hybridoma” in a sentence? Here are some example sentences to help you improve your vocabulary:
Stro-1 is a culture supernatant, monoclonal IgM, from Developmental Studies Hybridoma Bank, University of Iowa (Iowa City, IA, USA).
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
Following color development, the embryos were treated with 2 N NaOH for 1 hr, washed with PBSTx (phosphate buffered saline with 0.01% TritonX-100), blocked 1 hr in PBDTx (PBS, 1% DMSO, 0.01% TritonX-100 plus 2% sheep serum and 2 mg/ml BSA, then incubated 2 hr with anti-BrdU (monoclonal G3G4, Developmental Studies Hybridoma Bank).
The sections were rinsed again and incubated overnight at 4°C with the Rip primary antibody (Developmental Studies Hybridoma Bank, Iowa City, IA; diluted 1:4 in .1 M PBS).
The plates were dried, immersed in 0.5% polyisobutylmethacrylate in acetone, and sequentially incubated with Stx-1, anti-Stx-1 monoclonal antibody (purified from a hybridoma cell line, 13C4 (ATCC, Rockville, MD), as previously described [ 9 ] ), and 125I-goat anti-mouse IgG (DuPont NEN, Boston, MA).
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