Example sentences for: hybridoma

How can you use “hybridoma” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The plates were dried, immersed in 0.5% polyisobutylmethacrylate in acetone, and sequentially incubated with Stx-1, anti-Stx-1 monoclonal antibody (purified from a hybridoma cell line, 13C4 (ATCC, Rockville, MD), as previously described [ 9 ] ), and 125I-goat anti-mouse IgG (DuPont NEN, Boston, MA).

  • Additional primary antibodies used were zn-8 to detect Dmgrasp and zrf-1 (Developmental Studies Hybridoma Bank) and polyclonal rabbit anti Pax-2 (Berkeley Antibody Co.).

  • Monoclonal antibodies VCAM, STRO-1, ICAM-1(CD54), CD105, CD29, and MF20 were from Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, Iowa, United States); CD73, CD44, and ALCAM(CD166) were from BD Biosciences Pharmingen (San Diego, California, United States).

  • First, CD4 +T cells selectively proliferate in response to proteoglycan antigens [ 14 ] . Second, prevention of arthritis can be achieved by in vivo treatment with anti-CD4 monoclonal antibodies [ 15 ] . Third, arthritis can be transferred to naïve BALB/c or severe combined immunodeficient (SCID) mice using T and B cells from arthritic animals [ 16 17 18 ] . Fourth, a proteoglycan-specific T-cell hybridoma (T-helper-1 type) can induce arthritis in BALB/c mice [ 19 ] . Finally, CD4 +T cells from arthritic animals are resistant to activation-induced cell death [ 20 ] . These data suggest a breakdown of peripheral tolerance and accumulation of autoreactive T cells in the periphery.

  • Primary antibodies used: mouse anti-chicken Coll I, Coll III, FN, and TN (Hybridoma Bank, Iowa), rabbit anti-chicken TN (Chemicon, Temecula CA).


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