Example sentences for: hybridoma

How can you use “hybridoma” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Stro-1 is a culture supernatant, monoclonal IgM, from Developmental Studies Hybridoma Bank, University of Iowa (Iowa City, IA, USA).

  • NCAM: A mouse monoclonal antibody raised against the highly sialylated embryonic forms of the neural cell adhesion molecule was used (1:1, Developmental Studies Hybridoma Bank).

  • Following color development, the embryos were treated with 2 N NaOH for 1 hr, washed with PBSTx (phosphate buffered saline with 0.01% TritonX-100), blocked 1 hr in PBDTx (PBS, 1% DMSO, 0.01% TritonX-100 plus 2% sheep serum and 2 mg/ml BSA, then incubated 2 hr with anti-BrdU (monoclonal G3G4, Developmental Studies Hybridoma Bank).

  • with 20 μg anti-DNP-IgE (IgE hybridoma = H1 DNP-ε-26) [ 16 ] dissolved in 200 μl HMEM (Gibco-BRL, Gaithersburg, MD) with PIPES buffer (0.

  • Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.


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