Words similar to hybridized

Example sentences for: hybridized

How can you use “hybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
Filter lifts were taken from the grid of plaques and hybridized at 65°C overnight with labeled Drosophila head cDNA at 1 × 10 6cpm per ml hybridization buffer (50% formamide, 5x SSC, 0.1% Ficoll w/v, 0.1% PVP w/v, 0.1% BSA w/v, 0.1% SDS w/v, 0.2 μg ml -1salmon sperm DNA and 1 mM EDTA).
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.
For example, a probe specific for the 5' end of the CaCET1 gene (probe A in Figure 1) hybridized to a single 4.4 kbp Bgl II fragment after restriction digestion of total DNA from the parental diploid strain, whereas the heterozygote contained an additional 2.7-kbp fragment derived from scission at a novel Bgl II site located within the ARG4 component of the UAU1 insert of the disrupted cacet1::UAU1 allele (Figure 2A, lane P versus lane H).
Radioactivity in hybridized filters was imaged using a phosphorimager (Model 445 SI, Molecular Dynamics).