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Example sentences for: hybridizations
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The image for one of the hybridizations was selected to be the reference, and corresponding pixels in the other image were normalized by substituting them into a quadratic polynomial normalization function (of the pixel value), the coefficients of which were estimated by singular value decomposition (SVD) [ 10 ] . This quadratic model allows for some compensation in the event that the response of the phosphorimager changes between measurements.
Visual inspection of the intersection of a constant cutoff ratio with a distribution with many divergent genes demonstrates first, that the constant transition point intersects different distributions at different locations along the curve (Figure 3b; see also Figures 1, 2), and second, that there are divergent genes in some hybridizations which would be inappropriately deemed present instead of divergent because the constant transition point falls far to the left of the transition region (Figure 3b, Bongori hybridization).
Specifically, we examined individual replicate hybridizations of the A array from the worm developmental time course at each of 0 ( n = 4), 36 ( n = 3), 48 ( n = 4) and 60 ( n = 4) hours after synchronization of worm eggs by bleach, as well as a larger set of 13 hybridizations of all three arrays to samples ranging from oocytes to 2-week-old worms.
Microarray hybridizations were carried out according to previously published protocols [ 14, 37, 38, 39].
First, individual arrays were normalized using mean-log-centering and lowess procedures (as outlined in the Cell line self-self hybridizations section).
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