Words similar to hyaluronidase
Example sentences for: hyaluronidase
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The Sperm Adhesion Molecule 1 (SPAM1 or PH-20) is a glycosyl-phosphatidylinositol (GPI)-linked protein found on all mammalian spermatozoa that have been examined, and has been reported to play multiple roles in fertilization [ 1 ] . Although a recent gene targeting study indicates that in mice, which have four active non-somatic hyaluronidases, Spam1 is not essential for fertilization [ 2 ] ; this is unlikely to be the case in humans where SPAM1 is the only such hyaluronidase present.
5), which is typical only of sperm hyaluronidase.
Cumulus cells were removed by treating eggs for 3 min with 1 mg/ml hyaluronidase (Sigma, St. Louis, MO) in M199; eggs were washed through four 50-ml drops of M199 medium covered with mineral oil using a pulled , heat-polished, Pasteur pipette (employed in all experiments).
HASGE shows that epididymal SPAM1 has neutral hyaluronidase activity (Fig.
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.