Words similar to hsv-tk

Example sentences for: hsv-tk

How can you use “hsv-tk” in a sentence? Here are some example sentences to help you improve your vocabulary:

All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
It was derived from the plasmid pOS.DUP/DEL that contained the neomycin phosphotransferase (neo) gene that confers resistance to the pharmacological inhibitor G418 and also contains the Herpes Simplex Virus thymidine kinase (HSV-TK) that confers sensitivity to gancyclovir.
Cells containing Neo were selected by supplementing the ES cell medium with 300 μg/ml Geneticin (G418,Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).