Words similar to hsv-tk

Example sentences for: hsv-tk

How can you use “hsv-tk” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells containing Neo were selected by supplementing the ES cell medium with 300 μg/ml Geneticin (G418,Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
It was derived from the plasmid pOS.DUP/DEL that contained the neomycin phosphotransferase (neo) gene that confers resistance to the pharmacological inhibitor G418 and also contains the Herpes Simplex Virus thymidine kinase (HSV-TK) that confers sensitivity to gancyclovir.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).