Words similar to hsv-
Example sentences for: hsv-
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Virus sample numbers 1 through 15 were HSV-1, and 16 through 30 were HSV-2.
Three of the HSV-2 viruses, HSV-2 SB5, HSV-2 6652 and HSV-2 83D, had pol sequences consistent with wild type isolates found in GeneBank and were recently characterized [ 21 ] .
HSV-1 strain SC16 (wild type laboratory virus), HSV-2 SB5 (wild type laboratory virus), HSV-2 6652 (wild type clinical virus), HSV-2 83D (wild type clinical virus), HSV-2 6757 (high error mutant clinical virus) were described previously [ 21 ] and the HSV-1 PAA r5 (antimutator laboratory virus) utilized in this study was a kind gift of D. Coen (Harvard University, Boston, MA) [ 10 ] . HP66 (HSV-1 genome containing a LacZ insertion within the pol coding region) viral DNA was kindly provided by D. Coen (Harvard University, Boston, MA) [ 17 ] . HP66 viral stocks were prepared by transfecting HP66 viral DNA into PolB3 cells, which allow for virus propagation by expressing the viral polymerase.
A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (ori S ) SmaI fragment [ 5 ] . The plasmid pTK1 contains HSV-1 SC16 sequence coordinates 45,055-48,634 subcloned into the HindIII / EcoRI site of pUC19 (Invitrogen).
Mean PCV susceptiblities of the HSV-2 isolates (n = 105) from passages 1 through 7 are listed in Table 3. No resistant HSV-2 emerged among the total of 105 HSV-2 isolates tested from the three treatment groups (PCV IC
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