Words similar to hprt
Example sentences for: hprt
How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:
Moreover, because the transgene is introduced into the hprt locus its expression is dependent upon the inclusion of specific transcriptional regulatory elements and remains relatively unaffected by the local chromatin environment [ 2 ] . A major advantage of this targeted transgenic approach compared to traditional transgenics is that transgene expression is predictable so expression at ectopic sites is largely avoided.
To test this we generated a targeting vector to replace exon 1 of the mouse hprt gene with a neomycin phosphotransferase (neo) gene that conferred resistance to te pharmacological inhibitor G418 in ES cells (fig 1a).
The mice used in this study were first described in 1995 [ 31 ] . Disruption of the first coding exon with a promterless β-galactosidase/neomycin resistance fusion cassette [ 33 ] created a null allele for Lifr . AB1 ES cells, on a129S7/SvEvBrd- Hprt b - m 2background, were used for targeting Lifr . The mice were sent to the Induced Mutant Resource of the Jackson Laboratory in 1994, from where one heterozygous ( Lifr +/-) male was received in 1997 to re-establish the colony at the University of Washington.
We also tested whether TAR cloning with an ARS -containing vector and negative selection can be used to isolate a single copy gene from a complex genome, such as HPRT from the human genome.
The gene-expression levels obtained by RT-PCR were normalized to that of gene for the enzyme hypoxanthine-guanine phosphoribosyl transferase (HPRT) and expressed as the fold increase or decrease relative to that of the normal control pool.
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