Words similar to hprt

Example sentences for: hprt

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We next addressed whether embryos could be generated solely from F3 Hprt negative ES cells by aggregating these cells with tetraploid CD-1 embryos and allowing them to develop in utero until day 13 of gestation (El 3).
RT-PCR reactions using primers Hprt1a (5'-CCTGCTGGATTACATATAAGCACTG-3') and Hprt1b (5'-GTCAAGGGCATATCCAACAACAAAC-3') served as positive controls amplifying a 354 bp product from the ubiquitously expressed hprt transcript [ 15 ] .
Successful targeting of the transgene to the hprt locus in Hprt negative ES cells can be selected by growing transfected cells in HAT medium.
This vector, pMP8NEBΔLacZ, contains a 5' arm of homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 restriction endonuclease cut site.
Previously it has been shown that a targeting vector, pMP8, can correct the loss of Hprt activity in E14TG2a ES cells that lack exons 1 and 2 of the hprt gene [ 2 15 ] . The design of this vector implied that it should also be capable of correcting the hprt exon 1 deficiency in F3 cells and could, therefore, be used to introduce transgenes into the hprt locus by homologous recombination.