Words similar to hprt
Example sentences for: hprt
How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:
hprt replacement vector contains a 5' arm of hprt homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 cloning site.
The phenotype due to LIFR loss has remained consistent throughout the transition through three separate institutions and between the transition from, 129S7/SvEvBrd- Hprt b - m 2to greater than 96% C57BL/6.
Successful targeting of the transgene to the hprt locus in Hprt negative ES cells can be selected by growing transfected cells in HAT medium.
Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
Fig 1cshows that agouti F1 female mice contained both the mutant (paternal) and wild type (maternal) hprt alleles.