Words similar to hprt

Example sentences for: hprt

How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:

Successful targeting of the transgene to the hprt locus in Hprt negative ES cells can be selected by growing transfected cells in HAT medium.
The availability of F3 Hprt negative ES cells allows transgenic embryos to be derived directly from transgenic ES cells by aggregating the cells with tetraploid embryos and allowing them to develop in utero.
We screened 625 randomly picked transformants by PCR for the presence of HPRT , and 3 (~ 0.5%) of them carried all the HPRT exon sequences (Table 3).
However, pioneering work by Nagy et al has shown that embryos and mice can be derived directly from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . This suggests that controlled expression of transgenes encoding possible gain-of-function and developmental-lethal alleles could be achieved by generating embryos and/or mice from ES cells containing transgenes inserted into the hprt locus.
These data demonstrate that the hprt gene is not essential for the generation of embryos from ES cells by tetraploid aggregation and that F3 cells should could provide a suitable vehicle for the direct generation of transgenic mouse embryos.