Example sentences for: hprt

How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Consistent with this interpretation, Eggan et al have recently shown that viable offspring are more efficiently produced through tetraploid-mediated cloning from outbred ES cell line compared to inbred ES cell lines [ 14 ] . We, therefore, believed it likely that aggregation-compatible hprt negative ES cells could be generated by targeted mutation of the hprt gene in R1 ES cells.

  • In sum, the availability of tetraploid aggregation-competent ES cells that support targeting of transgenes to the hprt locus should facilitate the rapid analysis of transcriptional regulatory elements in vivo as well as the phenotypic characterization of alleles that are incompatible with normal development.

  • The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.

  • Thus, induction of RARβ2 is severely retarded and transient in lines A and B. In the same RNA samples, constant expression levels of HPRT were observed in differentiated and undifferentiated cells.

  • Unfortunately, the generation of mice and embryos by tetraploid aggregation from available Hprt negative ES cell lines has proven to be inefficient and unreliable (S.A.D.


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