Words similar to hprt

Example sentences for: hprt

How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:

We designed a TAR cloning vector pARS-HPRT carrying a 56-bp 5' HPRT -specific targeting hook and a 60-bp 3' HPRT -specific hook and inserted them into the promoter region of URA3 (see Methods).
Moreover, we demonstrate that it facilitates efficient targeting of transgenes to the hprt locus as well as the subsequent generation of clonal transgenic mouse embryos that express the transgene in a promoter-dependent manner.
These data demonstrate that the hprt gene is not essential for the generation of embryos from ES cells by tetraploid aggregation and that F3 cells should could provide a suitable vehicle for the direct generation of transgenic mouse embryos.
Fig 2dshows that all embryos contained a restriction fragment polymorphism that was indicative of deletion of hprt exon1.
The 3' arm of homology contains 1.8 kb of the human hprt gene that includes exon1 and 2.9 kb of mouse genomic sequence that includes exon 2. A detailed description of the pMP8 targeting vector, from which these sequences were derived, has been described previously [ 15 ] . A partial nucleotide sequence of pMP8NEBΔLacZ is available from S.A.D.