Words similar to hprt

Example sentences for: hprt

How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:

pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
We screened 625 randomly picked transformants by PCR for the presence of HPRT , and 3 (~ 0.5%) of them carried all the HPRT exon sequences (Table 3).
Moreover, because the transgene is introduced into the hprt locus its expression is dependent upon the inclusion of specific transcriptional regulatory elements and remains relatively unaffected by the local chromatin environment [ 2 ] . A major advantage of this targeted transgenic approach compared to traditional transgenics is that transgene expression is predictable so expression at ectopic sites is largely avoided.
This is an extremely efficient procedure because targeted clones have reconstituted the hprt gene and are selected on the basis of growth in HAT medium.
Details of the exon-specific primers for HPRT, TBP, and TAF II 135, are available upon request.