Words similar to hprt
Example sentences for: hprt
How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:
The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT . The hooks were amplified from HPRT yeast artificial chromosome (YAC) [ 6 ] with primer pairs HP-ARS-F and HP-ARS-R (Table 2) and cloned into the Xho I- Cla I sites of pARS-NK, yielding the vector pARS-HPRT.
Unfortunately, the generation of mice and embryos by tetraploid aggregation from available Hprt negative ES cell lines has proven to be inefficient and unreliable (S.A.D.
The 3' arm of homology was introduced into the unique Bcl1 site of the previous plasmid as a 7 Kb BamHI fragment from pMP1(SV129 origin) that contained murine hprt exons 2 and 3.
Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
The availability of F3 Hprt negative ES cells allows transgenic embryos to be derived directly from transgenic ES cells by aggregating the cells with tetraploid embryos and allowing them to develop in utero.
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