Example sentences for: hprt

How can you use “hprt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This vector, pMP8NEBΔLacZ, contains a 5' arm of homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 restriction endonuclease cut site.

  • We designed a TAR cloning vector pARS-HPRT carrying a 56-bp 5' HPRT -specific targeting hook and a 60-bp 3' HPRT -specific hook and inserted them into the promoter region of URA3 (see Methods).

  • RT-PCR reactions using primers Hprt1a (5'-CCTGCTGGATTACATATAAGCACTG-3') and Hprt1b (5'-GTCAAGGGCATATCCAACAACAAAC-3') served as positive controls amplifying a 354 bp product from the ubiquitously expressed hprt transcript [ 15 ] .

  • HPRT - hydroxyphosphoribosyl transferase

  • No wild type hprt allele was detected confirming that the embryos were generated entirely from F3 cells.


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