Words similar to hprt
Example sentences for: hprt
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The following primers were used: mouse CD36 5′ TGCTGGAGCTGTTATTGGTG and 3′ CATGAGAATGCCTCCAAACA, mouse beta actin 5′ ACCGTGAAAAGATGATGACCCAG and 3′ AGCCTGGATGGCTACGTACA, mouse HPRT1 5′ TGTTGTTGGATATGCCCTTG and 3′ TTGCGCTCATCTTAGGCTTT, human CD36 5′ GCTCTGGGGCTACAAAGATG and 3′ TAGGGAGAGATATCGGGCCT, human beta actin 5′ GATGAGATTGGCATGGCTTT and 3′ CACCTTCACCGTTCCAGTTT, and human HPRT1 5′ AAAGGACCCCACGAAGTGTT and 3′ TCAAGGGCATATCCTACAACAA.
This vector, pMP8NEBΔLacZ, contains a 5' arm of homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 restriction endonuclease cut site.
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
hprt replacement vector contains a 5' arm of hprt homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 cloning site.
Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
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