Words similar to hpo
Example sentences for: hpo
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Membranes were hybridized for 16 h at 65°C and briefly rinsed in low-stringency buffer containing 40 mM Na 2 HPO 4 (pH 7.2), 1 mM EDTA (pH 8.0) and 5% SDS at room temperature, followed by washing in low-stringency buffer at 65°C for 30 min.
To obtain nuclei, (4-6) × 10 7cells of each cell line grown in the presence and absence of 2.5 μM SAHA were washed twice in PBS, followed by suspension in 0.3 ml nuclear buffer consisting of 2 mM MgCl 2 , 5 mM K 2 HPO 4 , 0.1 mM EDTA, 1 mM PMSF, 20 μg/ml aprotinin, 20 μg/ml leupeptin, 0.1 mM Na 3 VO 4 and 5 mM β-glycerophosphate.
Fixed preparations were dropped onto clean glass slides and stained with 0.1 mg/ml acridine orange for 5 minutes, followed by a wash in running water for 2 minutes and a 1 minute incubation in Sorensen's buffer (67 mM KH 2 PO 4 + 67 mM Na 2 HPO 4 ). The wet slides were covered with glass coverslips and metaphases photographed immediately with a Zeiss Axioskop fluorescence microscope using the FITC filter and a 100× objective.
At the time of analysis, 1.5 ml of the transfected cells were transferred to eppendorf tubes, centrifuged and lysed in 320 μl of lysis buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, and 0.1% Triton X-100 at pH7).
The artificial saliva [ 17 ] was of the following composition: 0.1 L each of 25 mM K 2 HPO 4 , 24 mM Na 2 HPO 4 , 150 mM KHCO 3 , 100 mM NaCl, and 1.5 mM MgCl 2 . To this were added 0.006 L of 25 mM citric acid and 0.1 L of 15 mM CaCl 2 . The pH was then adjusted to 6.7 with NaOH or HCl and the volume made up to 1 L. To avoid bacterial growth, we added 0.05% by weight thymol to the artificial saliva.
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