Words similar to hpo
Example sentences for: hpo
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Embryos were enzymatically dechorionated with Pronase and extensively washed in embryo medium prior to injection Embryo medium = 13 mM NaCl; 4.2 mM NaHCO 3 ; 0.54 mM KCl; 0.025 mM Na 2 HPO 4 ; 0.044 mM KH 2 PO 4 ; 1.3 mM CaCl 2 ; 1 mM MgSO 4 ). Embryos were injected and maintained for 14 hours post fertilization on a bed of 0.7% agarose in embryo medium.
The nuclei prepared above were suspended in 10 ml of ice-cold TP buffer (10 mM Tris/HCl, pH 8.0, 10 mM Na 2 HPO 4 , 1 mM PMSF) containing 0.3 mg/ml heparin and 36 u/ml of DNase I. The suspension was gently stirred on a magnetic stirrer for 60 minutes at 4°C and 15 minutes at room temperature.
Between stimulations the tongue was rinsed with artificial saliva, which contained 2 mM NaCl, 5 mM KCl, 3 mM NaHCO3, 3 mM KHCO3, 0.25 mM CaCl2, 0.25 mM MgCl2, 0.12 mM K2HPO4, 0.12 mM KH2PO4, 1.8 mM HCl for pH 7. Because the precise content of mouse saliva is not published, we decided to use twice-diluted formula of the artificial saliva we have used in other species (monkey, pig, rat) [ 20 25 41 ] . It maintained the taste buds in good condition and gave us stable and reproducible nerve recordings for many hours.
At the time of analysis, 1.5 ml of the transfected cells were transferred to eppendorf tubes, centrifuged and lysed in 320 μl of lysis buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, and 0.1% Triton X-100 at pH7).
Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.
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