Words similar to hpo
Example sentences for: hpo
How can you use “hpo” in a sentence? Here are some example sentences to help you improve your vocabulary:
25 M Na 2 HPO 4 pH-7.
Membranes were washed twice at 65°C for 30 min in high-stringency buffer containing 40 mM Na 2 HPO 4 (pH 7.2), 1 mM EDTA (pH 8.0) and 1% SDS, before exposure to autoradiographic film.
2 HPO 4 , 1.4 mM KH 2 PO 4 , pH 7.4) using a BioRad Biospin P6 column.
The nuclei prepared above were suspended in 10 ml of ice-cold TP buffer (10 mM Tris/HCl, pH 8.0, 10 mM Na 2 HPO 4 , 1 mM PMSF) containing 0.3 mg/ml heparin and 36 u/ml of DNase I. The suspension was gently stirred on a magnetic stirrer for 60 minutes at 4°C and 15 minutes at room temperature.
Fixed preparations were dropped onto clean glass slides and stained with 0.1 mg/ml acridine orange for 5 minutes, followed by a wash in running water for 2 minutes and a 1 minute incubation in Sorensen's buffer (67 mM KH 2 PO 4 + 67 mM Na 2 HPO 4 ). The wet slides were covered with glass coverslips and metaphases photographed immediately with a Zeiss Axioskop fluorescence microscope using the FITC filter and a 100× objective.
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