Example sentences for: hpo

How can you use “hpo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PSS contained (mM): NaCl, 140; KCl, 4.7; 3-[N-morpholino] propane sulfonic acid (MOPS) 5; Na 2 HPO 4 , 1.2; CaCl 2 , 1.6; MgSO 4 , 1.2; D-glucose, 5.6; pH adjusted to 7.4 at 37°C.

  • Fixed preparations were dropped onto clean glass slides and stained with 0.1 mg/ml acridine orange for 5 minutes, followed by a wash in running water for 2 minutes and a 1 minute incubation in Sorensen's buffer (67 mM KH 2 PO 4 + 67 mM Na 2 HPO 4 ). The wet slides were covered with glass coverslips and metaphases photographed immediately with a Zeiss Axioskop fluorescence microscope using the FITC filter and a 100× objective.

  • Filters were hybridized at 65°C overnight with either labeled Drosophila body (minus head) or labeled Drosophila brain cDNA at 1 × 10 6cpm ml -1in Church and Gilbert buffer (7% SDS, 1 mM EDTA, 500 mM Na 2 HPO 4 , and pH to 7.2 with H 3 PO 4 [ 44]).

  • Membranes were hybridized for 16 h at 65°C and briefly rinsed in low-stringency buffer containing 40 mM Na 2 HPO 4 (pH 7.2), 1 mM EDTA (pH 8.0) and 5% SDS at room temperature, followed by washing in low-stringency buffer at 65°C for 30 min.

  • 2 HPO 4 pH-7.


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