Example sentences for: hpo

How can you use “hpo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PSS contained (mM): NaCl, 140; KCl, 4.7; 3-[N-morpholino] propane sulfonic acid (MOPS) 5; Na 2 HPO 4 , 1.2; CaCl 2 , 1.6; MgSO 4 , 1.2; D-glucose, 5.6; pH adjusted to 7.4 at 37°C.

  • After vortexing for 1 minute, 50 μL of saturated K 2 HPO 4 was added.

  • Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.

  • 2 HPO 4 , 1.4 mM KH 2 PO 4 , pH 7.4) using a BioRad Biospin P6 column.

  • At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.


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