Words similar to hpo

Example sentences for: hpo

How can you use “hpo” in a sentence? Here are some example sentences to help you improve your vocabulary:

The nuclei prepared above were suspended in 10 ml of ice-cold TP buffer (10 mM Tris/HCl, pH 8.0, 10 mM Na 2 HPO 4 , 1 mM PMSF) containing 0.3 mg/ml heparin and 36 u/ml of DNase I. The suspension was gently stirred on a magnetic stirrer for 60 minutes at 4°C and 15 minutes at room temperature.
2 HPO 4 pH-7.
It contained (in mM): 130 NaCl, 20 HEPES, 5 NaHCO 3 , 2.5 K 2 HPO 4 , 2 CaCl 2 , 1.2 MgSO 4 , 5.5 glucose, and 5 urea.
At the time of analysis, 1.5 ml of the transfected cells were transferred to eppendorf tubes, centrifuged and lysed in 320 μl of lysis buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, and 0.1% Triton X-100 at pH7).
To obtain nuclei, (4-6) × 10 7cells of each cell line grown in the presence and absence of 2.5 μM SAHA were washed twice in PBS, followed by suspension in 0.3 ml nuclear buffer consisting of 2 mM MgCl 2 , 5 mM K 2 HPO 4 , 0.1 mM EDTA, 1 mM PMSF, 20 μg/ml aprotinin, 20 μg/ml leupeptin, 0.1 mM Na 3 VO 4 and 5 mM β-glycerophosphate.