Words similar to hl-
Example sentences for: hl-
How can you use “hl-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pLD1A15SN:GFP-dynacortin plasmid was transformed into DH1 cells and the cells were grown in Hans' Enriched HL-5 with 7.5 μg/ml G418 [ 30 ] . For experiments with Texas Red-dextran, the fluorescent dextran was electroporated into cells.
In one report, ALDH1A1 protein was not detected by Western blot, but enzyme activity was detected using flow cytometry and a fluorescent aldehyde substrate [ 5 ] . In a second study, ALDH1A1 protein was detected using an ELISA assay and activity was detected using aldophosphamide as substrate [ 31 ] . In these two studies, designed to look at the relationship of ALDH1A1 activity to antitumor drug resistance, the levels of ALDH1A1 were low in HL-60 compared to other cell types, corresponding to the sensitivity of HL-60 cells to oxazaphosphorines.
The HL-60 human promyelocytic leukemia cell line responds in culture to sub-micromolar concentrations of retinoic acid ( all-trans and 13-cis ) by undergoing terminal differentiation to a granulocytic cell with the ability to phagocytose and reduce nitroblue tetrazolium [ 28 ] . The response to retinoic acid in HL-60 cells is dependent on the expression of the retinoic acid receptor RAR α [ 29 ] . The use of all-trans retinoic acid in differentiation therapy for patients with acute promyelocytic leukemia has resulted in prolonged, complete remissions when combined with cytotoxic chemotherapy, and may provide complete, long-term remissions as a single agent [ 30 ] .
2), and in nuclear extracts from SKW 6.4 (B lymphoid), KG-1 (T lymphoid), and HL-60 (monocytoid) cell lines (not shown).
ALDH activity has been reported in HL-60 cells using 4-hydroxynonenal as substrate [ 32 ] . However, since this molecule is such a poor substrate with ALDH1 compared to ALDH3, the measured activity likely represents only ALDH3 activity.
Loading...