Example sentences for: hl-

How can you use “hl-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In one report, ALDH1A1 protein was not detected by Western blot, but enzyme activity was detected using flow cytometry and a fluorescent aldehyde substrate [ 5 ] . In a second study, ALDH1A1 protein was detected using an ELISA assay and activity was detected using aldophosphamide as substrate [ 31 ] . In these two studies, designed to look at the relationship of ALDH1A1 activity to antitumor drug resistance, the levels of ALDH1A1 were low in HL-60 compared to other cell types, corresponding to the sensitivity of HL-60 cells to oxazaphosphorines.

  • A FLAG epitope was fused to the amino-terminus of MHCK-C at codon 2 using the vector pTX-FLAG [ 36 ] . The resulting plasmid, pTX-MKC2, was transformed into the cell line Ax2 and clonal cell lines were selected with G418 (8 μg/ml) in HL-5 medium.

  • 2), and in nuclear extracts from SKW 6.4 (B lymphoid), KG-1 (T lymphoid), and HL-60 (monocytoid) cell lines (not shown).

  • The pLD1A15SN:GFP-dynacortin plasmid was transformed into DH1 cells and the cells were grown in Hans' Enriched HL-5 with 7.5 μg/ml G418 [ 30 ] . For experiments with Texas Red-dextran, the fluorescent dextran was electroporated into cells.

  • Therefore, though low levels of ALDH1A1 activity have been reported in HL-60 cells in previous studies [ 5 31 ] , it appears that the differentiation observed in cells exposed to retinal alone or retinal plus DPAB was not mediated by ALDH1A1 oxidation of retinal to retinoic acid.


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