Words similar to hindiii
Example sentences for: hindiii
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The nucleotide sequence of the 5' flank was obtained from a HindIII subclone of c241.
The 9.7 kb αMHCLacZ expression cassette was constructed by inserting a 3.6 kb SalI/HindIII fragment from pLacF containing a lacZ gene that incorporates a nuclear localization signal into the SalI/HindIII site of an αMHC expression module.
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.
Exon 1a was located outside the sequenced regions of the genomic clone c241 [ 27 ] . To identify its location in c241, a HindIII restriction digest of c241 was subjected to southern blot hybridization analysis using a probe specific for exon 1a.
Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.