Words similar to hindiii

Example sentences for: hindiii

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To test this possibility, the PCR product was digested with HindIII.
Fig 3α shows that introduction of the LacZ transgene into the Hnf3 α locus was predicted to generate a 2.7 kb HindIII / EcoRV fragment that interacts with probe A. Fig 4shows that fifteen out of fifteen ES cell lines that were resistant to G418 had undergone homologous recombination and contained the LacZ transgene correctly targeted to the Hnf3 α locus.
A 2.4 kb HindIII fragment from pBS58 that contains the whole ftsZ gene was first subcloned into vector Litmus38 (New England Biolabs) whose EcoRI site has been deleted, and created a plasmid Litmus38(RI)+H3.
A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).