Words similar to hindiii
Example sentences for: hindiii
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pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
Promoter 1b was linked to the EGFP-F reporter using a HindIII/BamHI fragment that contained 5' flank, exons 1b and b', 3 KB of intron 1b', and the coding exon splice acceptor site preceded by 250 nucleotides of intron 1c.
A HindIII subclone of c241 was found with an insert identical in size with the interposed fragment (figure 2).
The nucleotide sequence of the 5' flank was obtained from a HindIII subclone of c241.
The PCR product was then cloned into the fs α 1S construct using HindIII and XhoI sites.
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