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Example sentences for: hindiii

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Exon 1a was located outside the sequenced regions of the genomic clone c241 [ 27 ] . To identify its location in c241, a HindIII restriction digest of c241 was subjected to southern blot hybridization analysis using a probe specific for exon 1a.
A 2.4 kb HindIII fragment from pBS58 that contains the whole ftsZ gene was first subcloned into vector Litmus38 (New England Biolabs) whose EcoRI site has been deleted, and created a plasmid Litmus38(RI)+H3.
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).
PCR and processing were as described above with the exception that the 5' human MYOC deletion products were cloned into a HindIII/Bsu36I digested pcDNA3.hMYOC wild-type vector.
The nucleotide sequence of the 5' flank was obtained from a HindIII subclone of c241.