Words similar to hincii

Example sentences for: hincii

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Each of the megaprimers was purified and subsequently amplified with GL1 primer (5' TGT ATC TTA TGG TAC TGT AAC TG 3'), using StuI B or HincII B plasmid as a template.
These deletion constructs define two regions as important for CD5 expression: a minimal/core promoter region (HincII to HinfI site) that is required for basal activity and a regulatory region necessary for the full CD5 expression (StuI to HincII) site in resting T cells.
For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.
Additional deletion from the HincII to the HinfI site at -67 further reduced the basal reporter activity down to the background level.
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.