Words similar to hincii

Example sentences for: hincii

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To determine whether the CD5X or CD5Y site is responsible for basal level expression, we generated three mutations encompassing the CD5X region (Xmut1, Xmut2, and Xmut3) and two mutations on the CD5Y region of the HincII B reporter plasmid (Ymut1 and Ymut2).
For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.
Additional deletion from the HincII to the HinfI site at -67 further reduced the basal reporter activity down to the background level.
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.
These deletion constructs define two regions as important for CD5 expression: a minimal/core promoter region (HincII to HinfI site) that is required for basal activity and a regulatory region necessary for the full CD5 expression (StuI to HincII) site in resting T cells.