Words similar to hincii
Example sentences for: hincii
How can you use “hincii” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine whether the CD5X or CD5Y site is responsible for basal level expression, we generated three mutations encompassing the CD5X region (Xmut1, Xmut2, and Xmut3) and two mutations on the CD5Y region of the HincII B reporter plasmid (Ymut1 and Ymut2).
Three potential transcription factor binding sites - CCAAT, κE2, and Ets - were previously identified by database sequence comparison in the 43 bp region described above (StuI to HincII) [ 25].
By constructing a deletion series of CD5 promoter constructs using luciferase as the reporter gene, we demonstrate that a 43 bp region between the StuI and HincII sites at -215 to -172 is necessary for normal, resting CD5 expression level in T cells.
Additional deletion from the HincII to the HinfI site at -67 further reduced the basal reporter activity down to the background level.
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.