Words similar to hincii
Example sentences for: hincii
How can you use “hincii” in a sentence? Here are some example sentences to help you improve your vocabulary:
By constructing a deletion series of CD5 promoter constructs using luciferase as the reporter gene, we demonstrate that a 43 bp region between the StuI and HincII sites at -215 to -172 is necessary for normal, resting CD5 expression level in T cells.
To determine whether the CD5X or CD5Y site is responsible for basal level expression, we generated three mutations encompassing the CD5X region (Xmut1, Xmut2, and Xmut3) and two mutations on the CD5Y region of the HincII B reporter plasmid (Ymut1 and Ymut2).
Each of the megaprimers was purified and subsequently amplified with GL1 primer (5' TGT ATC TTA TGG TAC TGT AAC TG 3'), using StuI B or HincII B plasmid as a template.
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.
For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.