Example sentences for: hindiii-xhoi

How can you use “hindiii-xhoi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).

  • The HindIII-XhoI fragment (nt 2228 to nt 2878) encompasing the II-III loop was subcloned into pCR 2.1 TOPO TA (Invitrogen, CA) and this plasmid was further used for PCR reactions.

  • Once amplified by PCR, the HindIII-XhoI digest was ligated into the T7-α 1S pSG5 vector using the same restriction sites.


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