Words similar to hindiii-xhoi
Example sentences for: hindiii-xhoi
How can you use “hindiii-xhoi” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).
The HindIII-XhoI fragment (nt 2228 to nt 2878) encompasing the II-III loop was subcloned into pCR 2.1 TOPO TA (Invitrogen, CA) and this plasmid was further used for PCR reactions.
Once amplified by PCR, the HindIII-XhoI digest was ligated into the T7-α 1S pSG5 vector using the same restriction sites.