Words similar to hgen

Example sentences for: hgen

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HGEN, HGEC and HPT were chosen for examination in this study because these cell types, at least in vitro , have been shown to be injured by Stx-1 [ 6 7 8 ] . Mesangial cells were not examined since Stx-1 has not been demonstrated to affect their viability [ 13 ] . Previous studies have separately examined the cytotoxic effect of Stx-1 on HGEN [ 8 ] , HGEC [ 10 ] , and HPT [ 9 ] , however this is the first study to directly compare Stx-1 sensitivity of these renal cell types.
Primary cultures of HGEN were obtained from Cell Systems (Kirkland, WA) and studied at the third passage.
HGEN were maintained in EGM2-MV media (Clonetics) which contains epidermal growth factor, hydrocortisone, vascular endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor-1, ascorbic acid, heparin and FBS.
The effect of 1, 2 or 7 days of exposure to physiologic or pharmacologic concentrations of progesterone, estradiol or testosterone on Gb3 expression by HGEN, HGEC or HPT was also examined.
These data indicate that, as suggested from the previous studies, within the limits of the cell culture system, HPT are the most sensitive to Stx-1 cytotoxicity of the three cell types, HGEC are slightly less sensitive, and HGEN are at least three orders of magnitude less sensitive than either HGEC or HPT.