Words similar to hdac-inhibiting
Example sentences for: hdac-inhibiting
How can you use “hdac-inhibiting” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell lines were treated with demethylating agent 5-Aza-2' deoxycytidine (Sigma) for five days at a concentration of 2 to 5 μM, HDAC-inhibiting agent n-butyrate at a final concentration of 5 μM for the last 24 hours or a combination of both.
Aberrant promoter methylation changes that occur in cancer are associated with transcriptional repression and loss of function of the methylated gene [ 9 ] . Since we found evidence of down-regulated expression of several methylated genes in CC cell lines, we tested to see if this down-regulation could be reactivated by methylation and HDAC-inhibiting agents.
2% of CC [ 13 ] . HIC1 is a zinc finger transcription factor that is transcriptionally silenced by promoter methylation in several types of human cancer [ 26 ] . Heterozygous Hic1 mouse with promoter methylation of wild type allele develop carcinomas, sarcomas and lymphomas, suggesting that this gene acts as a tumor suppressor gene [ 27 ] . HIC1 gene was down regulated in majority of the CC cell lines examined by us and this down-regulated expression was reactivated upon treatment with demethylating and HDAC-inhibiting agents.
The remaining five cell lines neither showed evidence of decreased mRNA nor any effect of demethylating or HDAC-inhibiting agents on expression levels.
Transcriptional silencing of genes resulting from DNA hypermethylation of CpG islands is reversed by treatment with the hypo-methylating agent 5-aza-2'-deoxycytidine and HDAC-inhibiting agent n-butyrate in a dose and duration-dependent manner.
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