Words similar to hcl

Example sentences for: hcl

How can you use “hcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

Fifty micrograms of the enzyme protein in 100 μL of buffer (50 mM Tris HCl, pH 7.8, 1 mM DTT and 400 mM NaCl) was carefully loaded onto 5 mL of 10-30% glycerol gradients prepared in the same buffer.
All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).
The cells were extracted for 5 min on ice with 200 μl of 2× detergent extraction buffer (2% of Triton X-100 or DOTMAC, 50 mM Tris-HCl, 10 mM EGTA, 200 mM NaCl, 2 mM MgCl 2 , protease inhibitor cocktail [20 μg/ml aprotinin, 20 μM E-64, 200 μM leupeptin, 200 μM p-amidinophenylmethanesulfonyl fluoride], 2 μM calyculin A, 10 mM sodium pyrophosphate, 2 μM staurosporine, 20 μM phalloidin, and 20 μg/ml taxol).
Each reaction contained 200 ng of RNA, reaction buffer [25 mM Tris-HCl (pH 8.3), 37.
5 ug of Fibrosarcoma PAI-1, or wtPAI-1 was denatured by boiling for 5 min in a buffer consisted of 0.5% SDS, 50 mM β-mercaptoethanol and 50 mM Tris-HCl, pH 7.4.