Example sentences for: hcl

How can you use “hcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The eluted phage are pooled, neutralized with 100 μl of 1 M Tris-HCl (pH 8.0).

  • Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.

  • This sample was loaded on a Superose-12HR gel filtration column from Pharmacia Biotech equilibrated with 40 mM Tris-HCl (pH7.

  • The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.

  • The samples were washed two times with buffer-1, two times with buffer-2 (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 0.1% Nonidet P40 and 0.05% sodium deoxycholate) and one time with buffer-3 (50 mM Tris-HCl, pH 7.5, 0.1% Nonidet P40 and 0.05% sodium deoxycholate).


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