Words similar to hcl

Example sentences for: hcl

How can you use “hcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

The reaction was allowed to proceed for 10 min at 37°C and stopped by addition of 300 μl of 1N HCl:methanol (1:1).
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
GAGs/polyanions purification was performed as follows: concentrated CM (15 ml) was dialyzed against buffer Q (50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 6 M Urea, 0.5% CHAPS), filtered through a 0.4 micron membrane and its soluble components subjected to anion-exchange column chromatography using Q-Sepharose Fast Flow (1 ml bed volume).
9, 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 15 mM KCl, 0.5 mM EDTA, 1 mM dithiothreitol, 10% glycerol, and 2 μg dIdC.
Polymerase chain reaction (PCR) was carried out in an 11-μl reaction volume containing 0.5 pM of each primer, 0.2 mM dNTP, 2 mM MgCl 2 , 5% glycerol, 0.275 U Taq polymerase, 50 mM KC1, 10 mM Tris-HCl pH 8.3, and 0.1 μg of genomic DNA.