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Example sentences for: hcl

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Polymerase activity of the HIV-1RT WT and insertion mutant enzymes was determined using two different template-primers: U-5PBS HIV-1 RNA and synthetic 49-mer U5-PBS DNA templates primed with the 17-mer PBS primer [ 36 ] . Assays were carried out in a 50 μL volume containing 50 mM Tris HCl, pH 7.8, 100 μg/mL bovine serum albumin, 5 mM MgCl 2 , 1 mM dithiothreitol, 60 mM KCl, 100 nM template-primer, 50 μM of each of the four dNTPs with one of them being 32P-labeled (0.
The membranes were blocked overnight at 4°C in TBST (10 mM Tris-HCl, 150 mM NaCl, 0.05% Tween; pH 8.0) containing 3% fat free dry milk.
The proteins were mixed with the 1 pmole of the labeled DNA substrates in 10 μl of 20 mM Tris-HCl pH 8.0, 100 μg/ml BSA, 12% glycerol and 0.5 mM EDTA.
PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.
2 μl of the single strand cDNA was amplified through 35 cycles (95°C for 1.0 min; 55°C for 1.0 min and 72°C for 2 min.) in a 50 μl reaction containing: 2 μM Neo and hnRNP A2/B1 specific primers, 10 mM Tris.HCl, pH (8.