Words similar to hccr

Example sentences for: hccr

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To determine whether yCCR4-yPOP2 interactions are evolutionarily conserved, we examined whether hCCR4 can bind hCAF1 and hPOP2 in a two-hybrid assay in mammalian cells.
The formerly identified "mCCR4", "hCCR4" [ 21 ] and nocturnin [ 22 ] proteins are grouped within the same family (called hereafter the nocturnin family, with the "mCCR4" and "hCCR4" proteins re-named m.nocturnin and h.nocturnin, and the xenopus nocturnin protein, xe.nocturnin).
To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
Interestingly, we found that hCCR4 also binds to the second human yPOP2 paralog, hCAF1.
Third, the ability of yCCR4 to bind mCAF1 and hPOP2 is conserved for the mammalian protein, since we could demonstrate, using two-hybrid assays and far-Western blot analyses, an in vivo and in vitro interaction between hCCR4 and hCAF1 or hPOP2/CALIF.