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Example sentences for: hccr

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This increase is dependent on the presence of the leucine-rich repeats within hCCR4, since deletion of four out of the five leucine-rich repeats -within pGal4-hCCR4(ΔLRR), see Materials and Methods- completely abolished the effect (Fig.
Construction of the deleted fusion protein in which four out the five hCCR4 leucine-rich repeats are removed (named pGal4-hCCR4(ΔLRR)) was performed in three steps.
After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
Finally, a GAL4 fusion vector expressing a murine paralog of hCCR4, i.
To confirm these data and gain some hints for the existence of a CCR4-NOT complex in mammals, we investigated whether the interaction between yCCR4 and yPOP2 in the CCR4-NOT complex is conserved for the human ortholog, hCCR4.