Words similar to hccr

Example sentences for: hccr

How can you use “hccr” in a sentence? Here are some example sentences to help you improve your vocabulary:

After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
Interestingly, we found that hCCR4 also binds to the second human yPOP2 paralog, hCAF1.
To verify that hCCR4 can bind directly to hCAF1, we performed a far-Western blot analysis (Fig.
Third, the ability of yCCR4 to bind mCAF1 and hPOP2 is conserved for the mammalian protein, since we could demonstrate, using two-hybrid assays and far-Western blot analyses, an in vivo and in vitro interaction between hCCR4 and hCAF1 or hPOP2/CALIF.
As a control, pGal4-hCCR4 alone, or in association with the pVP16-none plasmid, had no effect.