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Example sentences for: gtt

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Primer sequences were: IL-7 [ 65 ] : IL-7 (5') 5'ACT ACA CCC ACC TCC CGC A3'; IL-7 (3') 5'TCT CAG TAG TCT CTT TAG G3'; SCF [ 65 ] : SCF (5') 5'TCT TCA ACT GCT CCT ATT T3'; SCF (3') 5'ACT GCT ACT GCT GTC ATT C3'; TGFβ1 [ 66 ] : TGFβ1(5') 5'GCG GAC TAC TAT GCT AAA GAG G3'; TGFβ1(3') 5'GTT GTG TTG GTT GTA GAG GGC A3'; β-actin [ 67 ] : β-actin (5') 5'GGG TCA GAA GGA CTC CTA TG3'; β-actin (3') 5'GTA ACA ATG CCA TGT TCA AT3'.
For TNF-α the primers were sense primer, TCT GTC TAC TGA ACT TCG GGG T and antisense primer, TAG TTG GTT GTC TTT GAG ATC C. For COX-2 and TNF-α, 23 cycles and 26 cycles, respectively, fell within the linear range for each control and experimental sample.
The double stranded oligos used were as follows: for the Ets binding site on the CD5 promoter, 5' CGC GTA CAG GGA GGA AGT TGA CCT CGA 3'; for the oligonucleotides containing the CD5X region, 5' AGG CCT AAG TTG ACA GTT CAA CTT CAA ACA CTC GAG 3'; and for the CD5Y oligo, 5' AGG CCT CAC AGG CCC ACA CTG CCT GCT TCC CTG GAG 3'.
The competitor oligos are as follows: CD5 κE2, 5' GTA CCT AGG GGC AGG TGG TTT CAC GCG 3'; CD5 CK, 5' CCT CCA TTC TAA TTG GGG CAG GTG GTT TCA CAG G 3'; and CD5 KE, 5' AGG CCT GGG GCA GGT GGT TTC ACA GGG AGG AAG TTC TCG AG 3'.
80: C GCT ATC cTT GTT GGT GTT gAT GGT AGG NNN TAA NNN GAA CTG cCC GAG GAA CAT CAG NNN CAA GCG CGT CTG AAT AGG C (regions 2241-2244, 2266-2268, 2272-2274 are randomized, silent mutations are in lower case)