Words similar to gonadotropes
Example sentences for: gonadotropes
How can you use “gonadotropes” in a sentence? Here are some example sentences to help you improve your vocabulary:
Previously, we showed that RSG3 was expressed endogenously in rat pituitary cells and, when experimentally co-expressed together with GnRH receptors in a heterologous cell line (COS1), it suppressed GnRH-stimulated inositol trisphosphate production [ 9 ] . Here, those studies have been extended to include GnRH-stimulated LH secretion using adenovirus-mediated gene transfer of RGS3 complementary DNA into normal rat pituitary gonadotropes.
With respect to the G protein as a locus for modulation of GnRH receptor signaling, we reported earlier that experimental expression of RGS3 but not RGS1, 2, or 4 in GnRH receptor expressing COS-1 cells suppressed GnRH-stimulated IP 3 production [ 9 ] . In the current report, we present evidence strengthening the hypothesis of a regulatory role for RGS3 in GnRH receptor signaling: adenovirus-mediated expression of RGS3 in normal pituitary gonadotropes profoundly inhibited GnRH-stimulated LH secretion and 3H-inositol phosphate accumulation.
However, the calcium ionphore stimulated LH secretion in RGS3-expressing gonadotropes (Fig.
The pituitaries were dispersed with trypsin as described before [ 32 ] . The mono-dispersed cells were plated into poly-L-lysine coated 60 mm plastic Petri dishes in DMEM/10% gelding horse serum containing 10 -9M estradiol to optimize the GnRH-responsiveness of the gonadotropes [ 33 ] , and incubated overnight at 37°C in 8% CO 2 -92% air.
Because of the evidence that the signal transduction pathway for GnRH in gonadotropes involves the GnRH receptor, Gqα, phospholipase Cβ, and cellular IP 3 release [ 39 40 ] , we determined the effect of RGS3 on GnRH-stimulated LH secretion using adenoviral-mediated gene transfer in rat pituitary cells (Fig.
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