Words similar to fold-changes
Example sentences for: fold-changes
How can you use “fold-changes” in a sentence? Here are some example sentences to help you improve your vocabulary:
1 μg) can be used with our simplified amplification protocol to detect differential gene expression, (3) biological variation is larger than technical variation, (4) very low-level signals are prone to false positives and to less reliable fold-changes, false positives can be reduced by filtering out probe sets not reliably detected before statistical comparisons, and (5) using 4 independent biological samples is much better than using 3 samples in allowing detection of consistent changes and reducing false positives.
However, the output of such an experiment is often just a list of 'fold-changes' in gene-expression levels and so researchers face the question of whether their 'hits' can be trusted or not.
Genes expressed at these low levels often show high fold-changes because the denominator is so low (often near background), this points out the danger in emphasizing high fold-changes, rather than reproducible changes.
Figure 2shows the distribution of fold-changes in transcript levels in response to NGF.
Different methodologies and strategies for reducing noise include establishing an arbitrary global threshold for fold-changes [ 9 ] , noise-filtering look up tables [ 8 ] , normalization techniques to make microarrays comparable, such as using ANOVA methods to provide estimates of changes in gene expression that are corrected for potential confounding effects [ 10 11 ] , and using replicate experiments to estimate the variability in reported gene expression [ 12 ] . Applying fold change thresholds has been the most common method of reducing noise by filtering out the false positives [ 8 9 13 ] . However, much of the work done to date has focused on decreasing noise within the same generation and has not addressed the issue of comparability across generations.
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