Words similar to fluorescein-
Example sentences for: fluorescein-
How can you use “fluorescein-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although the structure of fluorescein is very similar to that of Oregon Green 488 (2', 7'-difluorofluorescein), fluorescein-SP did not behave in the same manner as Oregon Green 488-SP or unlabeled SP.
Of the conjugates that competed with [ 125I] SP, fluorescein-SP had the lowest binding affinity as compared to unlabeled SP.
Fluorescein-SP, Alexa 488-SP, BODIPY Fl-SP, fluorescein-SP, Oregon Green 488-SP or tetramethylrhodamine-SP was added at increasing concentrations, from 100 pM to 1 μM, to compete with the [ 125I] SP.
The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
2, the peak excitations of Alexa 488-SP, BODIPY Fl-SP, fluorescein-SP, Oregon Green 488-SP and tetramethylrhodamine-SP were 490 nm, 505 nm, 495 nm, 495 nm and 560 nm, respectively.