Example sentences for: fluorescein-

How can you use “fluorescein-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).

  • The evidence presented here suggests that four of the analogs, BODIPY Fl-SP, fluorescein-SP, Oregon Green 488-SP and tetramethylrhodamine-SP, are acting specifically at the SPR.

  • Fluorescein-SP, Alexa 488-SP, BODIPY Fl-SP, fluorescein-SP, Oregon Green 488-SP or tetramethylrhodamine-SP was added at increasing concentrations, from 100 pM to 1 μM, to compete with the [ 125I] SP.

  • Of the conjugates that competed with [ 125I] SP, fluorescein-SP had the lowest binding affinity as compared to unlabeled SP.

  • 0 nM) > fluorescein-SP (44.


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