Example sentences for: fluorescently

How can you use “fluorescently” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Detailed procedures for preparation of cDNA and fluorescently labeled cRNA, hybridization, staining, and scanning of gene chips and assay monitoring are outlined by Vahey et al . [ 16 ] . The platform chosen for global expression profile was the Rat Tox U34 Array (Affymetrix, Santa Clara, California), which contains probes for EST clusters and genes linked to a variety of toxic endpoints (total of 1031 probe sets including controls).

  • For each hybridization, 2 μg of each purified sample of polyadenylated RNA was reverse-transcribed in the presence of fluorescently labeled nucleoside triphosphates (Cy3-dUTP for reference sample, Cy5-dUTP for experimental sample).

  • Double stranded, fluorescently labelled (FAM, JOE, or NED) oligonucleotides (5' labelled oligonucleotide: CAGGAGATGCTGTTCGTAGG, unlabelled oligonucleotide: ACGAACAGCATCTCCT, supplier: Applied Biosystems) were annealed in 10 mM Tris pH 8.0, 10 mM NaCl, and 1 mM EDTA (3 min. at 94°C, cooling to 20°C within 15 minutes).

  • Fluorescently labeled cDNA was produced using a two-step procedure involving cDNA production from target RNA using a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide (aadUTP), followed by the second step involving chemical coupling of fluorescent dye (either Cy3 or Cy5) to the introduced amino moieties of the newly synthesized cDNA.

  • The basic methodology is to hybridize the fluorescently labeled genomic DNA of the strain of interest to the microarray along with the fluorescently labeled genomic DNA of a reference strain, typically the strain whose genome sequence the array was based on.


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