Words similar to fluorescein
Example sentences for: fluorescein
How can you use “fluorescein” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sections of fetal and residual maternal ovarian tissues were incubated for 30 min with 10% normal goat serum and for 1 h with primary antibodies (1 μg/ml), washed in two changes of PBS, incubated for 30 min with secondary goat antirabbit (F0382) or antimouse (F0257) immunoglobulin G-fluorescein isothiocyanate (FITC) (1:40), and washed in two changes of PBS.
AChRs were labeled with rhodamine (single label experiments) or fluorescein (double label experiments) conjugated αBGT (300 nM, Molecular Probes, T-1175/T-1176), and α-DG with a monoclonal antibody (1:1000, Upstate Biotechnology, 05-298, 45 minutes) followed by a secondary antibody (Rhodamine-Goat anti-mouse IgG, 1:100, Jackson Immuno Research Laboratories, 115-025-003, 45 minutes).
Sections were then incubated for 30 min at 4°C in fluorescein isothiocynate (FITC)-conjugated anti-rabbit antibody (Sigma) diluted 1:500 in blocking solution, and then washed three times for 5 min each in 1x PBS.
The investigators conjugated the Alexa fluorophores and other similar compounds, such as fluorescein, to different proteins to compare brightness and stability.
The p52 antibody was directly labelled with FITC (fluorescein isothiocyanate) and did not require a secondary antibody step.
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