Words similar to fluorescein
Example sentences for: fluorescein
How can you use “fluorescein” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cultures were then visualized using a Nikon Diaphot fluorescent microscope with a fluorescein filter and a 4x or 10x objective.
Fixed samples were immunostained with anti-Tag, PAb416 (Ab2, Oncogene Sciences, Mineola, NY), and fluorescein isothiocyanate (FITC) conjugated secondary antibody followed by RNase treatment and propidium staining at 50 μg/ml, as previously described [ 13 ] . Cells were excited with the 488 nm line of the argon laser of a Coulter ESP Elite Cytometer (Coulter Electronics, Miami, FL).
Optical filters designed for fluorescein and tetramethylrhodamine were used to separately visualize the monomer and J-aggregate forms, respectively.
Sections were then incubated for 30 min at 4°C in fluorescein isothiocynate (FITC)-conjugated anti-rabbit antibody (Sigma) diluted 1:500 in blocking solution, and then washed three times for 5 min each in 1x PBS.
AChRs were labeled with rhodamine (single label experiments) or fluorescein (double label experiments) conjugated αBGT (300 nM, Molecular Probes, T-1175/T-1176), and α-DG with a monoclonal antibody (1:1000, Upstate Biotechnology, 05-298, 45 minutes) followed by a secondary antibody (Rhodamine-Goat anti-mouse IgG, 1:100, Jackson Immuno Research Laboratories, 115-025-003, 45 minutes).
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