Example sentences for: fluorescein

How can you use “fluorescein” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sections of fetal and residual maternal ovarian tissues were incubated for 30 min with 10% normal goat serum and for 1 h with primary antibodies (1 μg/ml), washed in two changes of PBS, incubated for 30 min with secondary goat antirabbit (F0382) or antimouse (F0257) immunoglobulin G-fluorescein isothiocyanate (FITC) (1:40), and washed in two changes of PBS.

  • AChRs were labeled with rhodamine (single label experiments) or fluorescein (double label experiments) conjugated αBGT (300 nM, Molecular Probes, T-1175/T-1176), and α-DG with a monoclonal antibody (1:1000, Upstate Biotechnology, 05-298, 45 minutes) followed by a secondary antibody (Rhodamine-Goat anti-mouse IgG, 1:100, Jackson Immuno Research Laboratories, 115-025-003, 45 minutes).

  • Sections were then incubated for 30 min at 4°C in fluorescein isothiocynate (FITC)-conjugated anti-rabbit antibody (Sigma) diluted 1:500 in blocking solution, and then washed three times for 5 min each in 1x PBS.

  • The investigators conjugated the Alexa fluorophores and other similar compounds, such as fluorescein, to different proteins to compare brightness and stability.

  • The p52 antibody was directly labelled with FITC (fluorescein isothiocyanate) and did not require a secondary antibody step.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast