Words similar to fluorescein-conjugated
Example sentences for: fluorescein-conjugated
How can you use “fluorescein-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The effect of oxidation on pH-dependency of fluorescein-conjugated dextran was tested in vitro by the HRP/H 2 O 2 oxidant-generating system.
Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).
1 cells was measured by a dual excitation ratiometric measurement according to established procedures using the fluorescein-conjugated dextran [ 3 8 ] . Namely, cells were incubated with 2-5 mg/mL fluorescein-conjugated dextran for approximately 18 hours in culture medium.
To aid in the visualization of the periphery of the fibers, we simultaneously co-incubated each section with a rabbit anti-rat laminin primary antibody (Sigma, St Louis, Missouri) at a dilution of 1:2500, followed by a fluorescein-conjugated sheep anti-rabbit IgG secondary antibody.
Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] . The validation of lysosomal pH measurements was performed by the calibration curve using ionophore-clamped cells as shown in Figure 3. The ratio of emission fluorescence under excitation at 485 nm versus 440 nm (i.e.