Example sentences for: fluorescein-conjugated

How can you use “fluorescein-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells (100 μl) were incubated in the dark in the presence of fluorescein-conjugated annexin V (10 μl) and propidium iodide (10 μl) reagent for 20 min at room temperature.

  • Secondary antibodies were a fluorescein-conjugated goat anti mouse IgG (Boehringer Mannheim, IN) used at a dilution of 1:1000 and a fluorescein-conjugated donkey anti-rabbit IgG (Chemicon, CA) used at a dilution of 1:1000.

  • Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).

  • Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] . The validation of lysosomal pH measurements was performed by the calibration curve using ionophore-clamped cells as shown in Figure 3. The ratio of emission fluorescence under excitation at 485 nm versus 440 nm (i.e.

  • To aid in the visualization of the periphery of the fibers, we simultaneously co-incubated each section with a rabbit anti-rat laminin primary antibody (Sigma, St Louis, Missouri) at a dilution of 1:2500, followed by a fluorescein-conjugated sheep anti-rabbit IgG secondary antibody.


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