Words similar to fluorescein-conjugated
Example sentences for: fluorescein-conjugated
How can you use “fluorescein-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells (100 μl) were incubated in the dark in the presence of fluorescein-conjugated annexin V (10 μl) and propidium iodide (10 μl) reagent for 20 min at room temperature.
Secondary antibodies were a fluorescein-conjugated goat anti mouse IgG (Boehringer Mannheim, IN) used at a dilution of 1:1000 and a fluorescein-conjugated donkey anti-rabbit IgG (Chemicon, CA) used at a dilution of 1:1000.
Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).
Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] . The validation of lysosomal pH measurements was performed by the calibration curve using ionophore-clamped cells as shown in Figure 3. The ratio of emission fluorescence under excitation at 485 nm versus 440 nm (i.e.
To aid in the visualization of the periphery of the fibers, we simultaneously co-incubated each section with a rabbit anti-rat laminin primary antibody (Sigma, St Louis, Missouri) at a dilution of 1:2500, followed by a fluorescein-conjugated sheep anti-rabbit IgG secondary antibody.