Example sentences for: fibrosarcoma

How can you use “fibrosarcoma” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To confirm the validity of recombinant PAI-1 as a model to study the native inhibitor secreted from human fibrosarcoma cells, we compared the recombinant PAI-1 and the fibrosarcoma PAI-1 first by SDS-PAGE and immunoblot with anti-PAI-1 monoclonal antibodies.

  • In contrast, the specific activity of the fibrosarcoma PAI-1 was only 5 ± 3 U/ug before activation, and was only increased to 45 ± 16 U/μg after denaturing and refolding.

  • These results show that the wtPAI-1 could inhibit tPA or uPA at the same rate as the fibrosarcoma PAI-1.

  • Fibrosarcoma PAI-1, wtPAI-1 or its mutants were denatured with 6 M guanidine-hydrochloride (Gdn-HCl) (45 min at 37°C), followed by dialysis (4-16 hours at 4°C) against 50 mM sodium acetate, pH 5.6, containing 0.5 M NaCl and 0.01% Tween-20.

  • The high specific inhibitory activity of wt-PAI-1 could be due to the fact that it was secreted from insect High-5 cells cultured at 27°C, while the fibrosarcoma preparation of PAI-1 was obtained from the medium of fibrosarcoma cells cultured at 37°C [ 20 ] . Exposure to the higher temperature could accelerate the conversion of the inhibitor into its latent form.


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