Words similar to endonuclease
Example sentences for: endonuclease
How can you use “endonuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:
This vector, pMP8NEBΔLacZ, contains a 5' arm of homology consisting of 3.4 kb of mouse 5' hprt genomic DNA flanking a unique Not1 restriction endonuclease cut site.
They propagate by being transcribed from an internal RNA polymerase II promoter [ 15, 16], and then use their endonuclease and reverse transcriptase respectively to nick a target site and reverse transcribe L1 RNA, integrating the L1 into a new genomic locus [ 12, 17, 18]; this process is known as target-site-primed reverse transcription (TPRT).
There is evidence that the related sequence-specific R1 element endonuclease makes both cuts in the target DNA [ 18].
Although chromatid breakage requires an endonuclease activity, the cutter, directed by a Cbs-binding activity, no Cbs-dependent "binder/cutter" activity has been detected in vitro . The cutter could be an activity of telomerase itself [ 39 43 ] . Telomerase of both Tetrahymena and Euplotes has a single-strand endonuclease activity [ 45 43 ] . It can trim potential primers back, so that the 3' terminus pairs with template RNA [ 46 ] . Thus, while it may serve a 3' editing function, the existence of a nuclease activity in potential proximity to the site of chromatid breakage is provocative, suggesting that telomerase provides the
The endonuclease activity of DNase 1 is reduced many fold by integrating this loop from E. coli exonuclease III, but the mutant cleaves at abasic sites in DNA with low efficiency [ 29 ] . Thus additional residues in the APEs control specificity while still allowing a reasonable rate of phosphorolytic cleavage.
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