Words similar to endonucleases
Example sentences for: endonucleases
How can you use “endonucleases” in a sentence? Here are some example sentences to help you improve your vocabulary:
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
We have chosen to illustrate this approach using the multifunctional family of DNA repair proteins, the apurinic/apyrimidinic endonucleases (APEs), which have a clearly defined bacterial ancestor, E. coli exonuclease III (ExoIII), and are distantly related to several enzymes with varying substrate specificity.
Alignment of the yCCR4-related proteins with the Mg 2+-dependent endonucleases discloses another important feature : several residues (Leu 151, Arg 174 and Glu 300, indicated with an asterisk in Fig.
Inteins without this domain perform autocatalytic splicing [ 15 16 ] . Homing endonucleases and the process of homing have been more intensively studied in self splicing introns [ 17 ] , and the process is assumed to be similar for inteins.
Even small proteins may contain several individual domains that link them to different superfamilies [ 2 ] . While many endonucleases share a common active site that is highly conserved across many subfamilies, identifying residues that control substrate specificity requires sophisticated analysis that combines both sequence conservation and structural data [ 3 4 5 ] .
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