Words similar to endonucleases
Example sentences for: endonucleases
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Structural comparisons using the DALI program [ 20], indicated that the core fold shared by the KilA-N and APSES domains is more distantly related to the LAGLIDADG site-specific DNA endonucleases and the amino-terminal domain of the tRNA splicing endonucleases (TEN domain) (Figure 1b) [ 21].
Even small proteins may contain several individual domains that link them to different superfamilies [ 2 ] . While many endonucleases share a common active site that is highly conserved across many subfamilies, identifying residues that control substrate specificity requires sophisticated analysis that combines both sequence conservation and structural data [ 3 4 5 ] .
Inteins without this domain perform autocatalytic splicing [ 15 16 ] . Homing endonucleases and the process of homing have been more intensively studied in self splicing introns [ 17 ] , and the process is assumed to be similar for inteins.
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
We noticed, however, that, although the TEN domain is related to the LAGLIDADG endonucleases, the active-site residues are not preserved in the former, and the TEN domain probably functions as a RNA-binding domain rather than a nuclease.
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